PMID: 8610093Apr 2, 1996Paper

Spectroscopic recognition of guanine dimeric hairpin quadruplexes by a carbocyanine dye

Proceedings of the National Academy of Sciences of the United States of America
Q ChenR H Shafer

Abstract

Isolated guanine quadruplex structures have been described at high resolution both in solution and in the solid state. The existence of this unusual DNA structure in vivo and its biological significance remain to be determined. We describe the binding of 3,3'-diethyloxadicarbocyanine to dimeric hairpin guanine quadruplexes. This interaction results in a set of unique spectrophotometric signatures, none of which arises from binding to single strands or Watson-Crick duplexes. These unique signatures include a new absorbance peak (lambda max = 534 nm), an induced circular dichroism (lambda = 534-626 nm), a quenching of the dye fluorescence upon excitation with visible light, and strong energy transfer from DNA. This last effect provides the basis for detecting hairpin quadruplex structures in the presence of excess amounts of nonquadruplex DNA structures, such as single strands and Watson-Crick duplexes. The mechanism of quadruplex recognition by this dye is discussed, along with the possibility of using this dye as a probe for hairpin quadruplex structures in vitro and in vivo.

References

Oct 1, 1992·Biopolymers·N NageshD Chatterji
Apr 18, 1991·Nature·E H Blackburn
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·J R Williamson
Nov 30, 1994·Biochemical and Biophysical Research Communications·P Weisman-Shomer, M Fry
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Z LiuB K Tye

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Citations

Dec 18, 2001·Biopolymers·S Neidle, M A Read
Dec 18, 2001·Biopolymers·M A Keniry
Aug 10, 2000·Journal of Molecular Recognition : JMR·I Haq, J Ladbury
Nov 20, 2004·Chembiochem : a European Journal of Chemical Biology·Isabelle M DixonBernard Meunier
Sep 6, 2008·Investigational New Drugs·Blazej RubisMaria Rybczynska
Oct 3, 2003·Biochemical and Biophysical Research Communications·Souvik MaitiShantanu Chowdhury
Aug 19, 2003·European Journal of Pharmacology·Yan ZhangJing-Fen Qin
Mar 31, 2000·Trends in Pharmacological Sciences·H Han, L H Hurley
Sep 11, 2001·Bioorganic & Medicinal Chemistry Letters·S M KerwinP W Thomas
Jan 30, 2002·Bioorganic & Medicinal Chemistry Letters·Sean M KerwinPei Wang Thomas
May 9, 2000·Critical Reviews in Oncology/hematology·F LavelleP Mailliet
Nov 15, 2011·Bioconjugate Chemistry·Akash K Jain, Santanu Bhattacharya
Dec 10, 1998·Nature Medicine·J L Mergny, C Hélène
Jul 18, 2002·Nature Reviews. Drug Discovery·Stephen Neidle, Gary Parkinson
Oct 6, 2000·Journal of Biomolecular Structure & Dynamics·A L MikheikinA S Zasedatelev
Jul 11, 2001·Journal of Biomolecular Structure & Dynamics·R CaoB A Armitage
Mar 20, 2003·Journal of Biomolecular Structure & Dynamics·Xiao-Yan ZhangChunli Bai
Dec 11, 2007·Nucleosides, Nucleotides & Nucleic Acids·D MonchaudM-P Teulade-Fichou
Jan 15, 2002·Nucleic Acids Research·L Marcus WilhelmssonRichard N Zare
Jul 30, 2008·Nucleic Acids Research·Sattanathan Paramasivan, Philip H Bolton
Sep 9, 2008·Biochemistry. Biokhimii︠a︡·Bo ChenXiaochuan Liu
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·J L MergnyC Hélène
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·J F RiouJ L Mergny
Jan 5, 2001·Expert Opinion on Investigational Drugs·P J Perry, T C Jenkins
Mar 31, 1998·Journal of Pharmaceutical Sciences·C R MiddaughD R Casimiro
Mar 10, 2017·Chemistry : a European Journal·Ina BuchholzKlaus Weisz
Jun 19, 2004·Bioorganic & Medicinal Chemistry Letters·Bernard JuskowiakTadeusz W Hermann
Feb 12, 2008·Organic & Biomolecular Chemistry·David Monchaud, Marie-Paule Teulade-Fichou
Mar 6, 1999·The Journal of Biological Chemistry·W FuM P Mattson
Mar 13, 2018·Nucleosides, Nucleotides & Nucleic Acids·Jeremy E B McCallumBlake A Titterington
Jun 5, 2015·Organic & Biomolecular Chemistry·Aurélien LaguerreDavid Monchaud
Feb 27, 2013·Chembiochem : a European Journal of Chemical Biology·Balayeshwanth R VummidiNathan W Luedtke
Feb 1, 2008·ChemMedChem·Tian-miao OuLian-quan Gu
Mar 10, 2006·Organic & Biomolecular Chemistry·Markus KaiserMarie-Paule Teulade-Fichou

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