PMID: 2508754Oct 26, 1989Paper

Spectroscopic studies of cyanobacterial phycobilisomes lacking core polypeptides

Biochimica Et Biophysica Acta
P MaxsonA N Glazer

Abstract

Synechococcus sp. PCC 7002 (Agmenellum quadruplicatum PR6) genes encoding two highly conserved phycobilisome core polypeptides, a small linker polypeptide (LC8, apcC) and the allophycocyanin-B alpha-subunit (alpha APB, apcD), respectively, were interrupted by insertion of restriction fragments carrying the neomycin phosphotransferase gene of Tn5. The interrupted genes were used to transform Synechococcus sp. PCC 7002 to kanamycin resistance. The apcC- mutant assembled phycobilisomes lacking the LC8 polypeptide and the apcD- mutant assembled phycobilisomes lacking alpha APB. No other differences between the compositions of the mutant and wild-type phycobilisomes were detected. The apcC- strain grew about 25% more slowly than the wild-type, and its phycobilisomes dissociated more rapidly in 0.33 M Na/K-PO4 (pH 8.0) or in 0.75 M Na/K-PO4 at pH 8.0, at 40 degrees C, than did those of the wild-type. The phycobilisomes of this mutant were indistinguishable from those of the wild-type with respect to absorption and circular dichroism spectra, as well as time-resolved fluorescence emission. Steady-state emission spectra indicate a small decrease in long wavelength (680 nm) emission from the apcC- phycobilisomes and a complementary incr...Continue Reading

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Citations

Aug 14, 1992·Journal of Photochemistry and Photobiology. B, Biology·Y M GindtK Sauer
Dec 4, 2012·Biochimica Et Biophysica Acta·Ailie Marx, Noam Adir
Nov 3, 2010·Journal of Molecular Biology·Liron DavidNoam Adir
Mar 2, 1999·Journal of Structural Biology·R MacColl
Nov 11, 2018·Biochimica Et Biophysica Acta. Bioenergetics·Dmitry V ZlenkoIgor N Stadnichuk
Aug 9, 1990·Biochimica Et Biophysica Acta·R de LorimierS E Stevens
Sep 1, 1993·Microbiological Reviews·A R GrossmanJ L Collier

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