Spectroscopic studies on the inhibitory effects of ionic liquids on lipase activity

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Yunchang FanYan Li

Abstract

The effects of ionic liquids (ILs) on the lipase activity were studied by UV-Vis spectroscopy and the IL-lipase interaction mechanism at the molecular level was investigated by fluorescence technique. Experimental results indicated that the lipase activity was inhibited by ILs and the degree of inhibition highly depended on the chemical structures of ILs. The inhibitory ability of the Cl(-)- and Br(-)-based ILs increased with increasing the alkyl chain length in the IL cation. Thermodynamic parameters, enthalpy change (ΔH) and entropy change (ΔS) were obtained by analyzing the fluorescence behavior of lipase with the addition of ILs. Both ΔH and ΔS were positive suggesting hydrophobicity was the major driven force for the Cl(-)- and Br(-)-based ILs. For the BF4(-)-, CF3SO3(-)-, ClO4(-)- and N(CN)2(-)-based ILs, hydrogen bonding was the main driven force. For a more comprehensive understanding of the effects of ILs on lipase activity, the roles of hydrophobicity and hydrogen bonding must be considered simultaneously. A regression-based equation was developed to describe the relationship of the inhibitory ability of ILs and their hydrophobicity and hydrogen bonding ability.

References

Dec 7, 2010·Physical Chemistry Chemical Physics : PCCP·Marco KlähnPing Wu
Aug 8, 2012·Biomacromolecules·Lauri K J HauruHerbert Sixta

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Citations

Apr 11, 2017·ChemSusChem·Susana P F CostaM Lúcia M F S Saraiva
Oct 5, 2017·Arhiv za higijenu rada i toksikologiju·Marina Cvjetko BubaloVišnja Gaurina Srček
Apr 19, 2019·Bioprocess and Biosystems Engineering·Paloma Andrade Martins NascimentoValéria de Carvalho Santos-Ebinuma
May 16, 2019·Biotechnology Progress·Paloma A M NascimentoValéria C Santos-Ebinuma
Jan 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fátima A R MotaM Lúcia M F S Saraiva
May 7, 2021·Journal of Hazardous Materials·Sandra MaginaDmitry V Evtuguin
Mar 24, 2018·International Journal of Biological Macromolecules·Vítor Geniselli da Silva, Ruann Janser Soares de Castro
Jun 3, 2021·International Journal of Molecular Sciences·Ana R P GonçalvesCarla S G P Queirós
Nov 24, 2021·Chemical Society Reviews·Penghao WeiBing Yan

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