PMID: 8586971Nov 15, 1995Paper

Spectroscopic study of the interaction of aluminum ions with human transferrin

Journal of Inorganic Biochemistry
S TangP J Parsons

Abstract

Transferrin is the plasma protein responsible for transporting Fe3+ from the absorption to the utilization site. Interactions of apo- and holo-transferrin with Al3+ were studied by circular dichroism (CD), UV-visible, and fluorescence spectrometry. Binding of Al3+ to both metal-ion binding sites of apo-transferrin was confirmed by fluorescence studies. No interaction of Al3+ with holo-transferrin was observed, indicating that Al3+ cannot displace Fe3+ under the experimental conditions employed. An increase in tryptophan fluorescence (lambda max at 330 nm) by excitation at either 280 or 295 nm was observed after Al3+ interaction with apo-transferrin. There was no shift in wavelength of the fluorescence band of apo-transferrin after interaction with Al3+, but the intensity did increase. Since excitation at 295 nm is specific for tryptophan residues, tryptophan but not tyrosine must be responsible for the change in fluorescence intensity. Decreased fluorescence is the result of Fe3+ binding to apo-transferrin. The CD spectrum of apo-transferrin was slightly affected in the far UV by Al3+ binding, but a salient change was noted in the near UV at approximately 288 nm where tyrosine and tryptophan absorb. It is concluded that a small...Continue Reading

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Citations

Nov 3, 2004·Journal of the American Society for Mass Spectrometry·Mingxuan ZhangAnne B Mason
Aug 29, 1998·Clinical Biochemistry·G F van LandeghemP C D'Haese
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Murielle Rabiller-Baudry, Bernard Chaufer
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Arthur D TinocoAnn M Valentine
Oct 29, 2014·Journal of Toxicology·Christopher A ShawRobert M Davidson
Aug 23, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Elixabete RezabalJesus M Ugalde
Mar 12, 2003·Biochemistry·Miryana HémadiJean-Michel El Hage Chahine

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