Spectroscopic study on interaction of bovine serum albumin with sodium magnesium chlorophyllin and its sonodynamic damage under ultrasonic irradiation

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Jun WangXiangdong Zhang

Abstract

Sonodynamic therapy (SDT) is an attractive antitumor treatment for recent years. In this paper, sodium magnesium chlorophyllin (SMC) as a sonosensitizer combining with ultrasonic (US) irradiation to damage bovine serum albumin (BSA) has been investigated by fluorescence and UV-vis spectroscopy. The interaction of BSA with SMC was studied by the quenching of intrinsic fluorescence at varying temperature. The quenching constants (K(SV)), effective binding constants (K(A)), apparent association constants (K(a)) and binding site numbers were determined. The results indicated the quenching mechanism is a static procedure. Thermodynamic parameters show that the interactions involve hydrogen bonds, hydrophobic interactions, electrostatic interactions and complexations. The binding distance is 3.533 nm. The synergistic effect of SMC and ultrasound was estimated including the study of damage conditions. Synchronous fluorescence spectra indicate the damage to Trp residues is more serious. This paper may offer some valuable references for using spectroscopy method to study the application of chlorophyll derivatives in antitumor treatment.

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Citations

Jun 18, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jingqun GaoPingli Kang
Dec 24, 2010·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Bin LiuShu-Kun Xu
Feb 11, 2011·Journal of Biochemical and Molecular Toxicology·Dong YuanJianhua Zhu
Nov 5, 2011·Analytical Chemistry·Susmita DasIsiah M Warner
Dec 23, 2016·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Srijita DharMichael A Hill
May 29, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Yuanyuan YanJie Ou
Jul 27, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ling-Ling HeShu-Kun Xu

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