Spectrum of bacteriocin activity of Lactobacillus plantarum BS and fingerprinting by RAPD-PCR

International Journal of Food Microbiology
Francisco B ElegadoMarcelina B Lirazan

Abstract

The spectrum of antimicrobial activity of Lactobacillus plantarum BS against representative bacterial species was established through deferred assay and 'spot-on-lawn' assay using actively growing cells and partially purified bacteriocin extract, respectively. Only lactobacilli, pediococci, enterococci, bacilli and Listeria were inhibited from the test microorganisms. Slight bacteriocinogenic activity through 'spot-on-lawn' assay was detected against Staphylococcus aureus and Escherichia coli O157:H7. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis was used to compare the fingerprint of L. plantarum BS with other strains of L. plantarum. Using the 16S rRNA-based primer, P32, the bacteriocinogenic isolate exhibited identical RAPD-PCR fingerprints to L. plantarum ATCC 14917. Dendrograms derived from the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) were constructed to show the similarity relationships among the investigated strains based on RAPD-PCR analysis. Bands differentiating L. plantarum BS from L. plantarum ATCC 14917 were also identified by varying the annealing temperature.

References

Apr 1, 1996·International Journal of Systematic Bacteriology·F BringelJ C Hubert
Mar 29, 2000·Journal of Applied Microbiology·L NavarroC Torres
May 2, 2000·Applied and Environmental Microbiology·S M Cusick, D J O'Sullivan
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Citations

Jun 25, 2013·Applied and Environmental Microbiology·Sharath SrinivasiahK Eric Wommack
Nov 13, 2012·Annals of Microbiology·Tomasz GosiewskiPiotr B Heczko
Oct 25, 2014·Biotechnology Research International·Manas Ranjan SwainRizwana Parveen Rani
May 18, 2005·Journal of Food Protection·M Carmen ColladoYolanda Sanz

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