Speract, a sea urchin egg peptide that regulates sperm motility, also stimulates sperm mitochondrial metabolism

Biochimica Et Biophysica Acta
Juan García-RincónC Beltrán

Abstract

Sea urchin sperm have only one mitochondrion, that in addition to being the main source of energy, may modulate intracellular Ca(2+) concentration ([Ca(2+)]i) to regulate their motility and possibly the acrosome reaction. Speract is a decapeptide from the outer jelly layer of the Strongylocentrotus purpuratus egg that upon binding to its receptor in the sperm, stimulates sperm motility, respiration and ion fluxes, among other physiological events. Altering the sea urchin sperm mitochondrial function with specific inhibitors of this organelle, increases [Ca(2+)]i in an external Ca(2+) concentration ([Ca(2+)]ext)-dependent manner (Ardón, et al., 2009. BBActa 1787: 15), suggesting that the mitochondrion is involved in sperm [Ca(2+)]i homeostasis. To further understand the interrelationship between the mitochondrion and the speract responses, we measured mitochondrial membrane potential (ΔΨ) and NADH levels. We found that the stimulation of sperm with speract depolarizes the mitochondrion and increases the levels of NADH. Surprisingly, these responses are independent of external Ca(2+) and are due to the increase in intracellular pH (pHi) induced by speract. Our findings indicate that speract, by regulating pHi, in addition to [Ca(...Continue Reading

References

Jan 1, 1975·Experimental Cell Research·B BaccettiA G Burrini
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D F BabcockA Darszon
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S T SmileyL B Chen
Nov 12, 1990·Biochimica Et Biophysica Acta·M Mita, N Ueta
Dec 1, 1989·Trends in Pharmacological Sciences·P L Selby, H S Sherratt
Oct 1, 1986·The Journal of Physiology·R ChristenB M Shapiro
Mar 1, 1987·Developmental Biology·J García-SotoA Darszon
Apr 1, 1966·Experimental Cell Research·B A Afzelius, H Mohri
Nov 2, 1993·Biochimica Et Biophysica Acta·L Wojtczak, P Schönfeld
Jun 11, 1996·Biochemistry·C BeltránA Darszon
Feb 15, 1997·European Journal of Biochemistry·J D McGarry, N F Brown
Aug 7, 1998·Biochimica Et Biophysica Acta·P JezekK D Garlid
Jun 8, 2001·Biochemical and Biophysical Research Communications·T NishigakiA Darszon
Apr 20, 2002·Biophysical Journal·Shaohui HuangWatt W Webb
Jan 2, 2003·The Journal of Physiology·Esmeralda Rodríguez, Alberto Darszon
Apr 16, 2003·The Journal of Cell Biology·Chris D WoodMichael Whitaker
Jan 1, 2004·The Journal of Biological Chemistry·María F Cano AbadTullio Pozzan
Jan 23, 2004·Nature·Yuriy KirichokDavid E Clapham
Feb 11, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Herbert SchneckenburgerReinhard Sailer
Sep 15, 2004·Molecular Aspects of Medicine·Jean-Paul BonnefontJean Bastin
Nov 26, 2005·FEBS Letters·G Granados-GonzalezA Darszon
Feb 14, 2006·European Journal of Cardiovascular Nursing : Journal of the Working Group on Cardiovascular Nursing of the European Society of Cardiology·Sally Inglis, Simon Stewart
Apr 11, 2008·Physiology·György Szabadkai, Michael R Duchen
May 13, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Hiromutsu TominagaHideharu Hayashi
Nov 13, 2008·Biochimica Et Biophysica Acta·F ArdónA Darszon
May 6, 2009·Biochimica Et Biophysica Acta·Richard M Denton
Jan 19, 2010·Annual Review of Pathology·Douglas C WallaceVincent Procaccio
May 4, 2010·Developmental Biology·Adan GuerreroAlberto Darszon

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Citations

Jan 18, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Fabrizio GhiselliLiliana Milani
Apr 16, 2020·Physiology·Alberto DarszonClaudia L Treviño
Dec 3, 2021·Zygote : the Biology of Gametes and Early Embryos·Arlet Loza-HuertaCarmen Beltrán

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