Sperm and testis mediated DNA transfer as a means of gene therapy

Systems Biology in Reproductive Medicine
John ParringtonJoaquín Gadea


Assisted reproductive technologies (ART) have revolutionized the treatment of infertility. However, many types of infertility may still not be addressable by ART. With recent successes in identifying many of the genetic factors responsible for male infertility and the future prospect of whole individual human genome sequencing to identify disease causing genes, the possible use of gene therapy for treating infertility deserves serious consideration. Gene therapy in the sperm and testis offers both opportunities and obstacles. The opportunities stem from the fact that numerous different approaches have been developed for introducing transgenes into the sperm and testis, mainly because of the interest in using sperm mediated gene transfer and testis mediated gene transfer as ways to generate transgenic animals. The obstacles arise from the fact that it may be very difficult to carry out gene therapy of the testis and sperm without also affecting the germline. Here we consider new developments in both sperm and testis mediated gene transfer, including the use of viral vectors, as well as the technical and ethical challenges facing those who would seek to use these approaches for gene therapy as a way to treat male infertility.


Sep 1, 1992·Journal of Reproduction and Fertility·A CamaioniG Siracusa
Mar 1, 1992·Molecular Reproduction and Development·Marialuisa LavitranoC Spadafora
Nov 1, 1991·Molecular Reproduction and Development·D BachillerU Rüther
Jun 16, 1989·Science·M R Capecchi
Oct 20, 1989·Cell·Ralph L BrinsterR D Palmiter
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·B G BrackettH Koprowski
Oct 1, 1993·Molecular Reproduction and Development·A Nakanishi, A Iritani
Feb 1, 1993·Molecular Reproduction and Development·M FrancoliniC Spadafora
Feb 1, 1996·The Journal of Experimental Zoology·J G Patil, H W Khoo
Feb 1, 1997·Biology of Reproduction·K T Blanchard, K Boekelheide
Apr 7, 1997·Biochemical and Biophysical Research Communications·T MuramatsuJ Okumura
Apr 30, 1998·Molecular and Cellular Biochemistry·B S Shastry
Jul 21, 1998·Molecular Reproduction and Development·B MaioneA A Kiessling
May 15, 1999·Science·A C PerryRyuzo Yanagimachi
May 20, 1999·Molecular Reproduction and Development·L FarreJ E Rodríguez-Gil
Jan 5, 2000·The Journal of Experimental Zoology·Y YamazakiK Imoto
May 29, 2000·Molecular Reproduction and Development·H W Khoo
May 29, 2000·Molecular Reproduction and Development·H J Tsai
May 29, 2000·Molecular Reproduction and Development·M ShemeshY Stram
Jun 16, 2000·FEBS Letters·Makoto NaganoRalph L Brinster
Jan 5, 2001·Experimental Cell Research·R Carballada, P Esponda
Aug 2, 2001·Journal of Neuroscience Methods·T Wells, D A Carter
Sep 13, 2001·Molecular Reproduction and Development·T YonezawaM Nishihara
Jan 5, 2002·Theriogenology·R J Wall
Apr 12, 2002·Cloning and Stem Cells·R J Wall
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Masahito IkawaInder M Verma
Jul 24, 2002·FEBS Letters·Makoto NaganoRalph L Brinster
Aug 24, 2002·Biology of Reproduction·Kentaro YomogidaYoshitake Nishimune
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·F Kent HamraDavid L Garbers
Oct 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Marialuisa LavitranoGiancarlo Rossi
Feb 27, 2003·Biology of Reproduction·Maria P De Miguel, Peter J Donovan
May 3, 2003·Nature Reviews. Genetics·Clare E ThomasMark A Kay
Sep 6, 2003·Archives of Medical Research·Kevin R Smith
Oct 8, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Masahito IkawaInder M Verma
Jun 11, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Apr 16, 2005·Fertility and Sterility·Hiroki KubotaJohn Parrington
May 25, 2005·International Journal of Medical Sciences·Kevin R Smith
Jun 14, 2005·Theriogenology·Takehito KanekoPawel Pelczar
Jun 14, 2005·Developmental Biology·Masanobu ShojiNorio Nakatsuji
Aug 5, 2005·Biology of Reproduction·Ryota SuganumaStefan Moisyadi
Apr 21, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Arthur W NienhuisBrian P Sorrentino
May 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mito Kanatsu-ShinoharaTakashi Shinohara
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Jan Tesarik, Carmen Mendoza
Dec 16, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·P Mancheño-Corvo, P Martín-Duque
Mar 27, 2007·International Journal of Biological Sciences·Joan K Lunney
Jul 7, 2007·Archives of Andrology·Csilla Krausz, Claudia Giachini
Jul 13, 2007·Fertility and Sterility·Yoshiyuki KojimaKenjiro Kohri
Aug 9, 2007·Physiological Genomics·Frank Park
Aug 24, 2007·Reproduction, Fertility, and Development·Heiner Niemann, Wilfried A Kues
Sep 14, 2007·Archives of Andrology·Kevin CowardJohn Parrington
Feb 19, 2008·Nature Reviews. Genetics·Jonathan Kimmelman
Apr 9, 2008·Current Gene Therapy·Yoshiyuki KojimaKenjiro Kohri
Dec 24, 2008·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yidong Niu, Shulong Liang
Apr 28, 2009·Fertility and Sterility·Mohammad Hossein Nasr-EsfahaniMarziyeh Tavalaee
Oct 9, 2009·Biology of Reproduction·Chong LiTeruhiko Wakayama
Dec 17, 2009·Methods in Molecular Biology·Masumi Hirabayashi, Shinichi Hochi
Jan 28, 2010·Fertility and Sterility·Katherine L O'Flynn O'BrienAshok Agarwal
Mar 3, 2010·Genes & Development·Michael SnyderMark Gerstein
Mar 9, 2010·Annual Review of Biochemistry·Qinghua Liu, Zain Paroo
Mar 10, 2010·Current Drug Targets·Eishi AshiharaTaira Maekawa
Mar 12, 2010·The New England Journal of Medicine·James R LupskiRichard A Gibbs


Apr 25, 2012·Transgenic Research·Laurent BoulangerJean-Luc Vilotte
Dec 5, 2012·Advanced Biomedical Research·Nouri NayerossadatPalizban Abas Ali
Oct 29, 2010·Research in Veterinary Science·F A García-VázquezJoaquín Gadea
Aug 25, 2012·Cellular and Molecular Life Sciences : CMLS·Daniel ChevallierGeorges Pointis
Nov 30, 2013·The Urologic Clinics of North America·Boback M Berookhim, Peter N Schlegel
Nov 30, 2013·The Urologic Clinics of North America·James M Hotaling, Zamip Patel
Jan 22, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Natalia BarkalinaKevin Coward
Nov 10, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Natalia BarkalinaKevin Coward
Nov 1, 2018·Zygote : the Biology of Gametes and Early Embryos·Soleiman KurdMohammad Salehi

Related Concepts

DNA, Double-Stranded
Shuttle Vectors
Subfertility, Male
Y-Chromosome-Bearing Sperm
Genetic Therapy, Somatic
Subfamily lentivirinae
Gene Transfer Techniques

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.