Abstract
The binding of capacitated sperm to the egg's zona pellucida stimulates it to undergo the acrosome reaction, a process which enables the sperm to penetrate the egg. Mammalian sperm capacitation and the acrosome reaction require remodeling of actin filaments. An increase in phospholipase D (PLD)-dependent actin polymerization occurs during capacitation whereas the increase in sperm intracellular calcium after its binding to the egg causes very fast actin depolymerization prior to the acrosome reaction. Protein kinase A (PKA) and C (PKC) can both activate sperm PLD and actin polymerization under in vitro incubation, however under physiological conditions, actin polymerization depends primarily on PKA activity. We suggest that PKA indirectly activates phosphatidylinositol 4-kinase to produce phosphatidylinositol 4,5-bisphosphate which is a cofactor for PLD activation. In addition, activation of PKA during capacitation inactivates phospholipase C resulting in preventing PKC activation. It appears that PKA activation promotes sperm capacitation whereas too early activation of PKC during capacitation would jeopardize this process. Thus, a refined balance between the two pathways is required for optimal and sustained activation during...Continue Reading
References
Dec 17, 1993·Cell·H A BrownP C Sternweis
Feb 5, 2000·Cell·D RaucherT Meyer
Nov 4, 2000·Biology of Reproduction·M GarbiH Breitbart
Feb 27, 2003·Biology of Reproduction·Ephraim BrenerHaim Breitbart
Feb 21, 2004·Developmental Biology·G CohenH Breitbart
Mar 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Haim BreitbartSara Rubinstein
Citations
Nov 13, 2009·Journal of Molecular Histology·José R BardalesAntonio Villamarín
May 15, 2013·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·A O ShpakovN O Motovilova
Jun 23, 2010·BMC Systems Biology·Nicola BernabòBarbara Barboni
Jul 31, 2013·PloS One·Pnina HillmanHaim Breitbart
Aug 22, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Yadira BastiánAdela Mújica
Aug 17, 2010·Asian Journal of Andrology·Lei WangJia-Hao Sha
Jan 5, 2011·Asian Journal of Andrology·Haim Breitbart, Nir Etkovitz
May 31, 2013·Tsitologiia·K V DerkachA Iu Griaznov
Dec 6, 2012·Fertility and Sterility·Jun FuLinfang Wang
Mar 12, 2010·Molecular and Cellular Endocrinology·N BernabòB Barboni
Aug 31, 2010·Molecular Reproduction and Development·Juan J Fraire-Zamora, Richard A Cardullo
Nov 8, 2014·Computational and Structural Biotechnology Journal·Nicola BernabòMauro Maccarrone
May 15, 2007·Biology of Reproduction·Nir EtkovitzHaim Breitbart
Jul 7, 2007·Archives of Andrology·Melissa L VadnaisGary C Althouse
Jul 14, 2020·Andrology·Kun LiYa Ni
Sep 3, 2021·The Science of the Total Environment·Nathan TamRichard Yuen Chong Kong
Feb 17, 2009·Archives of Biochemistry and Biophysics·Aïda Abou-haila, Daulat R P Tulsiani