Sperm capacitation pretreatment positively impacts bovine intracytoplasmic sperm injection

Molecular Reproduction and Development
L AguilaRicardo Felmer

Abstract

The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is low compared to other species due in part to inadequate egg activation and sperm nucleus decondensation after injection. We hypothesized that this low efficiency is due to the lack of complete sperm capacitation, so we evaluated the effects of isobutylmethylxanthine (IBMX) and methyl-β-cyclodextrin (MβCD) on bovine sperm capacitation and on the preimplantation developmental potential of bovine embryos generated by ICSI. Treatment with IBMX and MβCD decreased sperm viability (between 13-30%); nevertheless, 0.4 mM IBMX and 1 mM MβCD increased (p < 0.05) capacitation metrics-that is, acrosome exocytosis, intracellular calcium level, plasma membrane fluidity, and tyrosine phosphorylation-compared to the control. After ICSI, embryos injected with IBMX- and MβCD-treated sperm showed similar cleavage to the untreated group (range 82-88%). Pronucleus formation rate was higher with MβCD-pretreatment (54%) compared to the control group (25%), and blastocyst rate was significantly improved with MβCD-pretreatment (24%) compared to the IBMX (18%) and control (17%) groups. Importantly, embryo quality-as assessed by the total number of cells, cell allocation, and apoptoti...Continue Reading

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Citations

Sep 5, 2018·Reproduction in Domestic Animals = Zuchthygiene·Camila Arroyo-SalvoRicardo Felmer
Jul 10, 2018·The Journal of Reproduction and Development·Hiroyuki Watanabe
Jul 29, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Luis ÁguilaRafael Fissore
Feb 13, 2021·Genes·Veena UnnikrishnanJacob Thundathil
Jan 22, 2021·The World Journal of Men's Health·Gabriela Hernández-SilvaMayel Chirinos

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