Sperm chromatin quality and DNA integrity in partial versus total globozoospermia

Andrologia
Ali Reza TalebiF Fesahat

Abstract

Globozoospermia is a severe form of teratozoospermia with low incidence in infertile patients, considered as one of the important causes of male infertility. The objective was to investigate the chromatin/DNA integrity as well as apoptosis in ejaculated spermatozoa of cases with partial or total globozoospermia. Fifty-seven semen samples were divided into three groups of partial globozoospermia (n = 17), total globozoospermia (n = 10) and normozoospermia (control; n = 30). Sperm chromatin condensation, DNA integrity and apoptosis were assessed using cytochemical assays. The results showed significant differences in sperm parameters of count and motility between two case groups versus controls. The percentages of spermatozoa with abnormal chromatin packaging and protamine deficiency were significantly higher in total and partial globozoospermic men compared to normozoospermic samples. Also, the rates of TUNEL-positive spermatozoa were significantly increased in both globozoospermic cases with respect to the control (18.3 ± 10.1 and 12.3 ± 9.2 versus 5.9 ± 3 respectively). However, no significant differences were noticed between two subgroups of patients with regard to sperm DNA denaturation, DNA fragmentation and apoptosis. Abno...Continue Reading

References

Aug 1, 1990·International Journal of Andrology·L RosenborgB Fredricsson
Feb 25, 1999·Mutation Research·R J PottsT M Jefferies
Feb 7, 2001·Human Reproduction·P SutovskyG Schatten
Mar 22, 2002·Biology of Reproduction·Denny SakkasDavide Bizzaro
Jul 20, 2002·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Luciano G NardoFiladelfo Nardo
Aug 2, 2002·Human Reproduction·Enzo VicariAldo E Calogero
Aug 21, 2003·Human Reproduction Update·A Agarwal, Tamer M Said
Feb 28, 2006·Fertility and Sterility·Kiran P NallellaAshok Agarwal
Sep 30, 2006·Human Reproduction Update·A H D M DamJ A M Kremer
Apr 22, 2008·The Urologic Clinics of North America·Ashok AgarwalEdmund Sabanegh
Dec 17, 2009·Journal of Assisted Reproduction and Genetics·Ryan T SchulteGary D Smith
Sep 25, 2010·Journal of Andrology·Anika H DamJan A Kremer
Dec 4, 2010·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·B SahuP Serhal
Jan 15, 2011·Reproductive Biomedicine Online·A PerrinF Morel
May 11, 2012·Andrologia·A PerrinM De Braekeleer

❮ Previous
Next ❯

Citations

Jun 18, 2019·Systems Biology in Reproductive Medicine·Elena MorettiCinzia Signorini
Nov 15, 2019·Andrologia·Farzaneh FesahatAshok Agarwal
Aug 5, 2020·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Y RaziM H Razi
Jul 25, 2020·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·M ImaniF Dehghanpour
Dec 15, 2018·Andrologia·Noushin MahdivandFatemeh Rahmani
Oct 27, 2020·Frontiers in Endocrinology·Robert John Aitken, Mark A Baker
Oct 17, 2020·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Amir Abdian AslAbolghasem Asadi-Saghandi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis