PMID: 28502148Jan 1, 2016Paper

Sperm DNA Fragmentation Index and Intracytoplasmic Sperm Injection Outcome in Egyptian Couples

The Egyptian Journal of Immunology
El-Geziry DEl-Dakhakhny M

Abstract

Sperm of infertile men mostly contain more DNA damage than their fertile counterparts. Sperm DNA damage might have a negative effect on the fertility potential of such patients. This study measures the percentage of spermatozoa with nuclear DNA fragmentation in semen samples used for intacytoplasmic sperm injection (ICSI), before and after sperm processing and investigates the association between sperm DNA fragmentation index (DFI) and ICSI outcome (embryo grade and clinical pregnancy). Sperm DFI was measured, by TUNEL, in fresh semen samples obtained from thirty male partners of infertile couples, seeking ICSI treatment. The mean sperm DFI before and after processing was 4.5% and 8.83% respectively. Sperm DFI increased significantly after processing (P < 0.001) regardless the type of processing used (P= 0.877). The correlation between sperm DFI, before and after semen processing, and clinical pregnancy was statistically insignificant (P=0.689 and P=0.631 respectively). DFI of sperm after semen processing showed a significant negative correlation with embryo grading at day 3 (r = - 0.232, P = 0.002). In conclusion, sperm DFI increases significantly after processing and correlates negatively with embryo grade.

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