Sperm-egg penetration assay assessment of the contraceptive effects of glycerol and egg yolk in rooster sperm diluents

Theriogenology
F M K AbouelezzJ Santiago-Moreno

Abstract

Glycerol (GLY) and egg yolk (EY) are good cryoprotectants of avian and mammalian sperm, but in birds, they strongly inhibit the eventual fertilization of ova. Using the sperm penetration (SP-holes) assay and fertility trials, the present study investigates (1) the possible mechanism by which this contraceptive effect occurs in chickens and (2) the maximum concentrations of GLY and EY tolerated by fresh rooster sperm. Seventy Black-Barred Andaluza hens (five per treatment) were inseminated four times (twice per week) with 0.1 mL of fresh semen from roosters of the same breed diluted 1:1 (v:v) with Lake and Ravie medium containing different concentrations of GLY or EY. No adverse effects on acrosome integrity, sperm motility, or viability were seen with any concentration of GLY or EY. The number of SP-holes on perivitelline layer samples taken from above the germinal disc became progressively lower at GLY concentrations of 1.5% or greater (P > 0.05). No holes caused by sperms were seen in unfertilized eggs. The corresponding fertility results showed similar reductions when the GLY concentration was 1.5% or greater. No changes in the number of SP-holes were seen with increasing EY concentrations (0%-7.5%), nor were any differences...Continue Reading

References

May 1, 1977·Poultry Science·B J Marquez, F X Ogasawara
May 1, 1977·Poultry Science·F D Westfall, B Howarth
Mar 1, 1978·British Poultry Science·P E Lake, J M Stewart
Feb 1, 1992·Cryobiology·R H Hammerstedt, J K Graham
May 1, 1987·Journal of Reproduction and Fertility·A Széll, J N Shelton
Oct 1, 1986·Poultry Science·L D BaconF X Ogasawara
Jan 1, 1984·British Poultry Science·P E Lake, O Ravie
Jan 1, 1981·British Poultry Science·P E LakeJ McAdam
Jul 1, 1996·Poultry Science·J J PhillipsJ K Graham
Aug 5, 2000·Animal Reproduction Science·W V Holt
Jul 21, 2009·Animal Reproduction Science·Phillip H PurdyHarvey D Blackburn
Oct 13, 2011·Reproduction in Domestic Animals = Zuchthygiene·J Santiago-MorenoJ L Campo

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