PMID: 3771636Oct 1, 1986Paper

Sperm exocytosis increases the amount of PH-20 antigen on the surface of guinea pig sperm

The Journal of Cell Biology
A CowanD G Myles

Abstract

Evidence has been presented that the PH-20 protein functions in sperm adhesion to the egg zona pellucida (Primakoff, P., H. Hyatt, and D. G. Myles, 1985, J. Cell Biol., 101:2239-2244). The PH-20 protein migrates from its original surface domain to a new surface domain after the acrosome reaction (Myles, D. G., and P. Primakoff, 1984, J. Cell Biol., 99:1634-1641). The acrosome reaction is an exocytotic event that results in insertion of a region of the secretory granule membrane, the inner acrosomal membrane (IAM), into the plasma membrane. After the acrosome reaction, PH-20 protein migrates to the IAM from its initial domain on the posterior head surface. We have now found a new dynamic feature of the regulation of PH-20 protein on the sperm surface; exocytosis increases the surface expression of PH-20 protein. After the acrosome reaction there is an approximately threefold increase in the number of PH-20 antigenic sites on the sperm surface. These new antigenic sites are revealed on the surface by insertion of the IAM into the plasma membrane. Our evidence indicates that before the acrosome reaction an intracellular population of PH-20 antigen is localized to the IAM. When migration of the surface population of the PH-20 prote...Continue Reading

References

Jan 1, 1985·Journal of Ultrastructure Research·G E Olson, V P Winfrey
Jan 1, 1974·Annual Review of Biochemistry·R A McRorie, W L Williams
Mar 1, 1973·Journal of Ultrastructure Research·F B Wooding
Nov 1, 1984·The Journal of Cell Biology·D G Myles, P Primakoff
Aug 1, 1983·Developmental Biology·P Primakoff, D G Myles
Dec 1, 1980·Developmental Biology·P PrimakoffA R Bellvé
Jan 1, 1982·The Journal of Cell Biology·D D SabatiniM Adesnik
Jul 25, 1955·The Journal of Biophysical and Biochemical Cytology·M H BURGOS, D W FAWCETT

❮ Previous
Next ❯

Citations

Sep 1, 1989·Journal of Reproductive Immunology·Y KawaiT Mimura
Dec 18, 2001·Animal Reproduction Science·S A Meyers
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y LinP Primakoff
Dec 31, 1997·Preparative Biochemistry & Biotechnology·J C Hall, C E Tubbs
Feb 29, 2000·Molecular Human Reproduction·A LenziM Picardo
Jan 1, 1991·Annals of the New York Academy of Sciences·D G Myles, P Primakoff
Jul 20, 2005·Archives of Andrology·H Zayas-PérezM Betancourt
May 14, 2005·Molecular Reproduction and Development·Guillaume MorinPierre Leclerc
Jun 18, 2003·Microscopy Research and Technique·P Talbot, Pramila Dandekar
Mar 25, 2004·Molecular Reproduction and Development·Jean S FlemingHelen D Nicholson
Jan 1, 1987·Annals of the New York Academy of Sciences·D G MylesP Primakoff
Aug 1, 1988·Gamete Research·L R Fraser, K K Ahuja
Jul 24, 2001·Biology of Reproduction·C LalancetteP Leclerc

❮ Previous
Next ❯

Related Concepts

Related Feeds