Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm

PLoS Genetics
Barbara LaurinyeczRita Sinka

Abstract

Drosophila melanogaster sperm reach an extraordinary long size, 1.8 mm, by the end of spermatogenesis. The mitochondrial derivatives run along the entire flagellum and provide structural rigidity for flagellar movement, but its precise function and organization is incompletely understood. The two mitochondrial derivatives differentiate and by the end of spermatogenesis the minor one reduces its size and the major one accumulates paracrystalline material inside it. The molecular constituents and precise function of the paracrystalline material have not yet been revealed. Here we purified the paracrystalline material from mature sperm and identified by mass spectrometry Sperm-Leucylaminopeptidase (S-Lap) family members as important constituents of it. To study the function of S-Lap proteins we show the characterization of classical mutants and RNAi lines affecting of the S-Lap genes and the analysis of their mutant phenotypes. We show that the male sterile phenotype of the S-Lap mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives. Our work shows that S-Lap proteins localize and accumulate in the paracrystalline material of the major mitochon...Continue Reading

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Citations

Jul 23, 2019·PLoS Genetics·Christopher Ellison, Doris Bachtrog
Oct 31, 2019·Traffic·Celestin N MudogoChristian Betzel
Sep 24, 2020·Cell Discovery·Shuqing ChenYongping Huang
Aug 23, 2020·International Journal of Molecular Sciences·Irina V OgnevaOleg I Orlov
Aug 25, 2021·Genome Biology and Evolution· MrinaliniNalini Puniamoorthy
Oct 19, 2021·Frontiers in Cell and Developmental Biology·Elham AlzyoudRita Sinka

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Methods Mentioned

BETA
RNA-Seq
transmission electron microscopy
electron microscopy
transgenic
PCR
acetylation
X-ray
phase

Software Mentioned

iTEM
UCSF Chimera
FlyAtlas
Axoneme
Clustal Omega
PAVA
MitoFates
ProteinProspector
Modeller

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