Sperm maturation in rhesus monkey: changes in ultrastructure, chromatin condensation, and organization of lipid bilayer

The Anatomical Record
P Sivashanmugam, M Rajalakshmi

Abstract

The changes in ultrastructure, lipid organization, chromatin decondensation, and denaturation of rhesus monkey spermatozoa during epididymal maturation were studied. This study would provide background information that would be useful to evaluate adverse effects, if any, caused by the use of contraceptive agents. Adult sexually mature rhesus monkeys were castrated under ketamine anesthesia. The epididymis was divided into initial segment, caput, corpus, and cauda epididymides. To study changes in lipid organization of the sperm plasma membrane during epididymal transit, spermatozoa from different epididymal segments and ejaculated spermatozoa were exposed to merocyanine 540 (MC 540). The changes in chromatin denaturation and decondensation were assessed by using the nucleic acid-specific fluorochromes, acridine orange, and ethidium bromide, respectively, prior to and after exposure to dithiothreitol (DTT). Testicular spermatozoa (approximately 40%) showed localization of MC 540 mainly in the midpiece, whereas remaining sperm did not localize MC 540. Spermatozoa from the initial segment of the epididymis showed uniform distribution of MC 540 localization in the head and midpiece. A pattern of localization of MC 540 similar to ma...Continue Reading

References

Apr 1, 1976·General and Comparative Endocrinology·M R Prasad, M Rajalakshmi
Apr 1, 1976·Experimental Cell Research·M L MeistrichW J Barcellona
Nov 1, 1991·Journal of Reproduction and Fertility·J Seligman, R Shalgi
Mar 1, 1991·The Anatomical Record·R A Kumar, D M Phillips
Nov 1, 1985·The American Journal of Anatomy·K K Ahuja
Dec 1, 1972·The American Journal of Anatomy·R YanagimachiG L Nicolson
Jun 1, 1972·Journal of Reproduction and Fertility·B L Gledhill
May 1, 1984·The Journal of Cell Biology·D E Wolf, J K Voglmayr
Feb 1, 1981·Molecular and Cellular Biology·P L WilliamsonR A Schlegel
May 1, 1981·Archives of Andrology·J K Koehler
Mar 1, 1982·The Journal of Cell Biology·E L Bearer, D S Friend
Jan 1, 1994·Molecular Reproduction and Development·S YossefiL M Lewin
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Apr 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·L MASTROIANNI, W A MANSON

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Citations

Jun 15, 1999·International Journal of Andrology·G van Der HorstS Hendricks
Jan 10, 2002·Journal of Biosciences·R S SharmaD A Jeyaraj
Feb 27, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·K FaisalM A Akbarsha
Apr 9, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·M MarchlewiczB Wiszniewska
Oct 30, 2014·Reproduction, Fertility, and Development·Xin LiuFu-Jun Liu
Sep 19, 2003·Acta histochemica·Daniela MeggiolaroBruno Ferrandi
Jan 24, 2006·Molecular and Cellular Endocrinology·Joël R Drevet

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