Sperm-mediated gene transfer in the silkworm Bombyx mori

Archives of Insect Biochemistry and Physiology
Y Shamila, S Mathavan

Abstract

Plasmid DNA containing the CAT reporter gene was injected into the testis of V instar silkworm larvae. The persistence, expression, and transmission of the injected DNA were monitored in the injected individuals till eclosion as well as in the progeny. The DNA injected into the testis persisted extrachromosomally during the entire period of metamorphosis and was also transferred into the egg via sperm during fertilization. Injected plasmids were rescued from the moths that emerged from the injected larvae and also from the eggs laid by the moths that copulated with injected males. Positive signals for CAT assay in the experimental samples suggested that the injected DNA was internalized in the testicular cells and sperm. The persistence, expression, and transmission of the DNA injected into the testes indicate that sperm-mediated gene transfer is possible in the silkworm, Bombyx mori.

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Citations

Dec 21, 2000·Insect Molecular Biology·K O RobinsonB H Smith
May 29, 2000·Molecular Reproduction and Development·Y Shamila, S Mathavan
Jan 28, 2003·Molecular Reproduction and Development·Marialuisa LavitranoEraldo Seren

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