Sperm membrane fatty acid composition in the Eastern grey kangaroo (Macropus giganteus), koala (Phascolarctos cinereus), and common wombat (Vombatus ursinus) and its relationship to cold shock injury and cryopreservation success

Cryobiology
R R MillerS D Johnston

Abstract

Marsupial spermatozoa tolerate cold shock well, but differ in cryopreservation tolerance. In an attempt to explain these phenomena, the fatty acid composition of the sperm membrane from caput and cauda epididymides of the Eastern grey kangaroo, koala, and common wombat was measured and membrane sterol levels were measured in cauda epididymidal spermatozoa. While species-related differences in the levels of linolenic acid (18:3, n-6) and arachidonic acid (20:4, n-6) were observed in caput epididymal spermatozoa, these differences failed to significantly alter the ratio of unsaturated/saturated membrane fatty acids. However in cauda epididymidal spermatozoa, the ratio of unsaturated/saturated membrane fatty acids in koala and kangaroo spermatozoa was approximately 7.6 and 5.2, respectively; substantially higher than any other mammalian species so far described. Koala spermatozoal membranes had a higher ratio of unsaturated/saturated membrane fatty acids than that of wombat spermatozoa (t = 3.81; df = 4; p < or = 0.02); however, there was no significant difference between wombat and kangaroo spermatozoa. The highest proportions of DHA (22:6, n-3), the predominant membrane fatty acid in cauda epididymidal spermatozoa, were found in...Continue Reading

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Citations

Jun 2, 2006·Reproduction, Fertility, and Development·Rhett McCleanStephen D Johnston
Mar 2, 2010·Reproduction, Fertility, and Development·N A Czarny, J C Rodger
Mar 14, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Lindsay A HoganStephen D Johnston
May 5, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K E WaterhouseR R Miller
May 12, 2009·Cryobiology·Yeng Peng ZeeStephen D Johnston
Oct 28, 2008·Theriogenology·John C RodgerStephen D Johnston
Apr 28, 2007·Cryobiology·Rhett V McCleanStephen D Johnston
Aug 9, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Yilmaz AksoyAsuman Ozkan
Nov 23, 2006·Journal of Microbiological Methods·Patrice FrancoisJacques Schrenzel
Dec 21, 2005·Reproduction, Fertility, and Development·C MacCallum, S D Johnston
May 5, 2021·Reproduction, Fertility, and Development·R R MillerB Nixon
Aug 7, 2021·Antioxidants·Eliana Pintus, José Luis Ros-Santaella
Aug 20, 2021·Proteomics·David A Skerrett-ByrneBrett Nixon

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