Sperm metabolism is altered during storage by female insects: evidence from two-photon autofluorescence lifetime measurements in bedbugs

Journal of the Royal Society, Interface
Klaus ReinhardtKarsten König

Abstract

We explore the possibility of characterizing sperm cells without the need to stain them using spectral and fluorescence lifetime analyses after multi-photon excitation in an insect model. The autofluorescence emission spectrum of sperm of the common bedbug, Cimex lectularius, was consistent with the presence of flavins and NAD(P)H. The mean fluorescence lifetimes showed smaller variation in sperm extracted from the male (tau m, τm = 1.54-1.84 ns) than in that extracted from the female sperm storage organ (tau m, τm = 1.26-2.00 ns). The fluorescence lifetime histograms revealed four peaks. These peaks (0.18, 0.92, 2.50 and 3.80 ns) suggest the presence of NAD(P)H and flavins and show that sperm metabolism can be characterized using fluorescence lifetime imaging. The difference in fluorescence lifetime variation between the sexes is consistent with the notion that female animals alter the metabolism of sperm cells during storage. It is not consistent, however, with the idea that sperm metabolism represents a sexually selected character that provides females with information about the male genotype.

References

Dec 14, 1985·British Medical Journal·M G HullK M Desai
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·A D Stutt, M T Siva-Jothy
Apr 23, 2002·Nature Reviews. Genetics·Timothy R Birkhead, Tommaso Pizzari
Nov 9, 2002·Science·Gary T Miller, Scott Pitnick
Oct 7, 2003·Annual Review of Microbiology·James A Imlay
Dec 12, 2003·Proceedings. Biological Sciences·Klaus ReinhardtMichael T Siva-Jothy
Oct 1, 2005·Lasers in Surgery and Medicine·Maor EichlerRachel Lubart
Jan 7, 2006·Photochemistry and Photobiology·Beata KoziolBarbara Plytycz
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Melissa C SkalaNirmala Ramanujam
Jan 26, 2008·The Quarterly Review of Biology·Klaus Reinhardt
Apr 10, 2008·Biological Reviews of the Cambridge Philosophical Society·Michael Werner, Leigh W Simmons
Jul 24, 2008·The International Journal of Developmental Biology·Susan S Suarez
Aug 30, 2008·Evolution; International Journal of Organic Evolution·Klaus ReinhardtMichael T Siva-Jothy
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Joël WhiteEtienne Danchin
Mar 4, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Carsten HilleCarsten Dosche
Apr 4, 2009·Journal of Biophotonics·Karsten König
Sep 18, 2009·Biology of Reproduction·Polina V DanshinaDeborah A O'Brien
Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Klaus ReinhardtMichael T Siva-Jothy
Apr 2, 2010·Chemical Reviews·Mikhail Y Berezin, Samuel Achilefu
Jul 1, 2010·Optics Express·Hans Georg BreunigKarsten König
Sep 22, 2010·Journal of Cellular Physiology·Haruhiko MiyataRichard A Cardullo
Jan 27, 2012·Proceedings. Biological Sciences·Anne-Cécile Ribou, Klaus Reinhardt
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Alison Pischedda, William R Rice
Nov 20, 2012·Molecular & Cellular Proteomics : MCP·Alexandra AmaralRafael Oliva
Jun 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Stefan LüpoldMollie K Manier
Oct 8, 2013·Evolution; International Journal of Organic Evolution·Cedric K W TanStuart Wigby
May 16, 2014·The EMBO Journal·Kira T PateMarian L Waterman
Apr 7, 2015·Trends in Ecology & Evolution·Teri J Orr, Patricia L R Brennan
May 23, 2015·Asian Journal of Andrology·Robert J AitkenBrett Nixon

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Citations

May 25, 2016·Microscopy Research and Technique·J AdurH F Carvalho
Dec 4, 2016·Arthropod Structure & Development·Klaus ReinhardtKarsten König
Nov 19, 2019·Biological Reviews of the Cambridge Philosophical Society·Scott PitnickSteve Dorus
Apr 9, 2020·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Biz R Turnell, Klaus Reinhardt

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Methods Mentioned

BETA
Fluorescence

Software Mentioned

SPCI mage
SPCImage

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