Sperm Microbiota and Its Impact on Semen Parameters

Frontiers in Microbiology
David BaudMilos Stojanov

Abstract

Compared to its female counterpart, the microbiota of the male genital tract has not been studied extensively. With this study, we aimed to evaluate the bacterial composition of seminal fluid and its impact on sperm parameters. We hypothesized that a dysbiotic microbiota composition may have an influence on sperm quality. Semen samples of 26 men with normal spermiogram and 68 men with at least one abnormal spermiogram parameter were included in the study. Samples were stratified based on total sperm count, spermatozoa concentration, progressive motility, total motility and spermatozoa morphology. Microbiota profiling was performed using 16S rRNA gene amplicons sequencing and total bacterial load was determined using a panbacterial quantitative PCR. Semen samples broadly clustered into three microbiota profiles: Prevotella-enriched, Lactobacillus-enriched, and polymicrobial. Prevotella-enriched samples had the highest bacterial load (p < 0.05). Network analysis identified three main co-occurrence modules, among which two contained bacteria commonly found in the vaginal flora. Genera from the same module displayed similar oxygen requirements, arguing for the presence of different ecological niches for bacteria that colonize semen...Continue Reading

References

Sep 26, 2000·Fertility and Sterility·E CottellC Barry-Kinsella
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Mar 11, 2008·International Journal of Andrology·Iuri B IvanovViktor A Gritsenko
May 30, 2009·Science·Elizabeth A GriceJulia A Segre
Mar 23, 2010·Journal of Clinical Microbiology·Marcela Zozaya-HinchliffeMichael J Ferris
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jacques RavelLarry J Forney
Aug 17, 2010·Bioinformatics·Robert C Edgar
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Oct 3, 2012·PLoS Computational Biology·Jonathan Friedman, Eric J Alm
Nov 13, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Reet Mändar
Sep 3, 2013·Fertility and Sterility·Dongsheng HouChen Xu
Feb 14, 2014·Biology Letters·Taichi A Suzuki, Michael Worobey
Oct 22, 2014·Nature Reviews. Urology·Fabrícia GimenesMarcia E L Consolaro
Apr 15, 2015·Research in Microbiology·Reet MändarJaak Truu
Feb 2, 2017·International Journal of Urology : Official Journal of the Japanese Urological Association·Reet MändarJaak Truu
Jun 20, 2017·Current Protocols in Mouse Biology·Alexis RapinNicola L Harris
Aug 29, 2017·Trends in Microbiology·Jessica A YounesMariya I Petrova
Feb 16, 2018·Cell Host & Microbe·Melis N AnahtarDouglas S Kwon

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Citations

Aug 3, 2019·American Journal of Reproductive Immunology : AJRI·Kristian LeisegangAshok Agarwal
Apr 30, 2020·Proceedings. Biological Sciences·Sara BellinviaOliver Otti
May 31, 2019·Journal of the International AIDS Society·Salim S Abdool KarimBrent L Williams
Jul 17, 2020·Journal of Assisted Reproduction and Genetics·Andrea CarossoChiara Benedetto
Nov 17, 2019·Nature Reviews. Urology·Signe AltmäeReet Mändar
Apr 3, 2020·Current Opinion in Urology·Scott D LundyRanjith Ramasamy
Jul 14, 2019·International Urology and Nephrology·Joseph K M LiChi-Fai Ng
Oct 20, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Rudi L VerspoorNina Wedell
Sep 13, 2020·Microbiome·George GolovkoYuriy Fofanov
Aug 15, 2020·Reproduction in Domestic Animals = Zuchthygiene·Chetna GangwarSuresh Dinkar Kharche
Feb 12, 2021·Frontiers in Cellular and Infection Microbiology·Fenghao ZhangTingtao Chen
Mar 23, 2021·Current Sexual Health Reports·Vadim OsadchiySriram V Eleswarapu
Sep 17, 2019·Revista clínica española·T Requena, M Velasco
Nov 13, 2019·Theriogenology·Gaël EvenChristophe Audebert
Apr 23, 2021·BMC Infectious Diseases·Heidi TuominenSamuli Rautava
Dec 18, 2020·The Journal of Infectious Diseases·Laurel A LagenaurThomas P Parks
Aug 5, 2021·Reproductive Biomedicine Online·Nerea M MolinaSigne Altmäe
May 18, 2021·Revista Clínica Espanõla·T Requena, M Velasco
Nov 18, 2021·Critical Reviews in Microbiology·Richard CariñoMasahiro Umezaki

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Datasets Mentioned

BETA
PRJNA509076

Methods Mentioned

BETA
PCR
Illumina sequencing

Software Mentioned

SparCC
ANOSIM
R
LEfSe
join
fastq
Quantitative Insights into Microbial Ecology ( QIIME
CASA SCA
QIIME
igraph

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