Sperm midpiece apoptotic markers: impact on fertilizing potential in in vitro fertilization and intracytoplasmic sperm injection

Human Cell
Joanna Talarczyk-DesolePiotr Jędrzejczak

Abstract

The aim of this study was to investigate the relationship between apoptotic markers present in human spermatozoa, namely phosphatidylserine translocation (PST) from the inner to the outer layer of the cytomembrane and the active form of caspase-3 (c3) versus the fertilizing potential of male gametes in conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) models. A total of 116 male patients treated with their partners for infertility underwent basic semen analysis and an assessment of the presence of PST and the active c3 in sperm using flow cytometry. Forty patients underwent IVF, group A, while 76 patients underwent ICSI, group B. The fertilizing potential of the gametes was measured as the percentage of oocytes with pronuclei present after either procedure. PST and active c3 were identified in vital gametes, mainly in the midpiece area. Concentration, motility, morphology, and viability of spermatozoa strongly negatively correlated with both markers. In group A, a negative correlation between both markers and the success rate of conventional IVF was observed (r = -0.4, p = 0.04 for PST; r = -0.4, p = 0.02 for active c3, respectively). In group B, the success rate of ICSI did not correlate wit...Continue Reading

References

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Citations

Jun 3, 2011·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Iori KisuYasunori Yoshimura
Oct 21, 2018·Reproductive Biology and Endocrinology : RB&E·Pamela Tello-MoraBetzabet Quintanilla-Vega
Dec 31, 2019·Journal of Toxicology·Manuel Sánchez-GutiérrezJeannett Alejandra Izquierdo-Vega
Feb 9, 2021·Human Reproduction Update·Magalie BoguenetPascale May-Panloup

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Statistica
CellQuest Pro

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