Sperm mitochondrial DNA depletion in men with asthenospermia

Fertility and Sterility
Shu-Huei KaoYau-Huei Wei

Abstract

To determine the content of sperm mitochondrial DNA (mtDNA) in patients with asthenospermia or with poor sperm motility. Analysis of the content of mtDNA as the ratio between the amount of mtDNA and nuclear DNA by using a new concurrent polymerase chain reaction. University hospital infertility center. Eighty-six men who sought infertility therapy. Moving characteristics of sperm were examined with a computer-assisted semen analyzer. Sperm samples were classified into two groups, one with asthenospermia and the other with normal moving characteristics. The content of mtDNA in sperm was determined by polymerase chain reaction. We analyzed the mitochondrial mass by MitoTracker Green staining and analyzed DNA content with propidium iodide staining by flow cytometry. A decrease in sperm mtDNA content was detected in patients with asthenospermia or with poor sperm motility (<20% motility). The relative mtDNA contents in the asthenospermia and normal groups were 7.2 +/- 1.3 (mean +/- SD, n = 23) and 74.1 +/- 2.0 (n = 29), respectively. Lower intensities of propidium iodide staining were detected in patients with asthenospermia or poor motility, but there was no significant difference in MitoTracker Green staining between the sperm wi...Continue Reading

References

Dec 1, 1992·The Journal of Pediatrics·M R MazziottaG Silvestri
Nov 1, 1982·Developmental Biology·G S MichaelsP J Laipis
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·F Ankel-Simons, J M Cummins
Oct 23, 1997·American Journal of Human Genetics·G ManfrediE A Schon
Apr 17, 1998·Human Genetics·J N SpelbrinkC Van den Bogert
Feb 2, 1999·Pediatrics·J Marin-GarciaA Perez-Atayde
Mar 5, 1999·Science·D C Wallace
Jun 27, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·Q Y SunH Schatten
Oct 23, 2001·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A RantanenN G Larsson
Jun 20, 2002·Human Genetics·Cyrille BarthélémyAnne Lombès
Jun 25, 2002·Genomics·Yves TourmenPascal Reynier
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Petras P DzejaAndre Terzic
Aug 1, 2002·Molecular Human Reproduction·John SpiropoulosPatrick F Chinnery
Aug 23, 2002·The New England Journal of Medicine·Marianne Schwartz, John Vissing
Dec 21, 2002·Biology of Reproduction·Carmen Díez-SánchezManuel J López-Pérez
Mar 5, 2003·Human Reproduction·P May-PanloupP Reynier

❮ Previous
Next ❯

Citations

May 9, 2012·Journal of Assisted Reproduction and Genetics·Maria San GabrielArmand Zini
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shi-Ming LuoQing-Yuan Sun
Mar 17, 2006·The British Journal of Ophthalmology·K K Abu-Amero, T M Bosley
Feb 24, 2006·Asian Journal of Andrology·Giorgio Cavallini
Oct 23, 2012·Mitochondrion·Afshan N Malik, Anna Czajka
Sep 24, 2014·International Journal of Environmental Research and Public Health·Constanza MorénGlòria Garrabou
Nov 19, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Shi-Ming LuoQing-Yuan Sun
Mar 20, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Moustafa A A El-TaiebMichael Marberger
Jan 27, 2007·Chemico-biological Interactions·Dar-Bin ShiehHo-Yuan Chang
Sep 29, 2011·International Journal of Andrology·P PiomboniV Zara
Sep 4, 2015·Annals of the New York Academy of Sciences·William T Y Lee, Justin St John
Sep 12, 2006·Gynécologie, obstétrique & fertilité·P May-PanloupP Reynier
Apr 3, 2016·Reproduction in Domestic Animals = Zuchthygiene·M OrsztynowiczD Lechniak
Sep 4, 2008·Ophthalmic Genetics·Khaled K Abu-AmeroThomas M Bosley
Mar 28, 2018·European Journal of Human Genetics : EJHG·Yeşerin YıldırımAslıhan Tolun
Jan 4, 2021·Journal of Assisted Reproduction and Genetics·Swati PandruvadaTomer Avidor-Reiss
Feb 9, 2021·Human Reproduction Update·Magalie BoguenetPascale May-Panloup
Jun 3, 2021·International Journal of Molecular Sciences·Isidora M StarovlahSilvana A Andric
Aug 28, 2021·Antioxidants·Zofia E MadejaNatalia Rozwadowska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.