Sperm morphology and preparation method affect bovine embryonic development

Journal of Andrology
Anneke H WaltersFrancis C Gwazdauskas

Abstract

This study was conducted to evaluate the effect of sperm separation methods of semen samples collected from bulls subjected to scrotal insulation on embryonic development after in vitro fertilization (IVF) and to determine whether IVF results would be affected by various heparin concentrations. Morphologically abnormal semen samples were obtained and cryopreserved from Holstein bulls following scrotal insulation for 48 hours. Standard protocols using the Percoll gradient (90%/45%) method and the swim-up method were used to separate spermatozoa fractions in experiment I. The pellet (A(p)) and the 45% layer (B(p)) were isolated from the Percoll separation, while for the swim-up separation, the supernatant (A(s)) and the interphase (B(s)) were isolated. The overall blastocyst rate for our laboratory control semen was 23.1 +/- 2.1% for Percoll separations (A(p) and B(p)) and 18.2 +/- 2.0% for swim-up (A(s) and B(s)) separations. This rate was higher (P <.01) than the rate observed for the semen from the bull that had the greatest response to scrotal insult 5 days prior to the insult, when it was 9.2 +/- 2.1% for the Percoll separation and 20.7 +/- 2.3% for the swim-up separation, while semen from 27 days after scrotal insulation (D...Continue Reading

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Citations

Dec 21, 2012·Animal : an International Journal of Animal Bioscience·M GaulyC Wrenzycki
Feb 23, 2020·Molecular Reproduction and Development·Gry Brandt Boe-HansenMichael McGowan
Feb 26, 2021·Journal of Thermal Biology·Arnaldo Algaranhar GonçalvesOtávio Mitio Ohashi
Apr 3, 2007·Animal Reproduction Science·T A A KhalifaTh Papanikolaou

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