Sperm Oxidative Stress Is Detrimental to Embryo Development: A Dose-Dependent Study Model and a New and More Sensitive Oxidative Status Evaluation

Oxidative Medicine and Cellular Longevity
Letícia S de CastroMayra E O A Assumpção

Abstract

Our study aimed to assess the impact of sperm oxidative stress on embryo development by means of a dose-dependent model. In experiment 1, straws from five bulls were subjected to incubation with increasing H2O2 doses (0, 12.5, 25, and 50 μM). Motility parameters were evaluated by Computed Assisted System Analysis (CASA). Experiment 2 was designed to study a high (50 μM) and low dose (12.5 μM) of H2O2 compared to a control (0 μM). Samples were incubated and further used for in vitro fertilization. Analyses of motility (CASA), oxidative status (CellROX green and 2'-7' dichlorofluorescein diacetate), mitochondrial potential (JC-1), chromatin integrity (AO), and sperm capacitation status (chlortetracycline) were performed. Embryos were evaluated based on fast cleavage (30 h.p.i.), cleavage (D = 3), development (D = 5), and blastocyst rates (D = 8). We observed a dose-dependent deleterious effect of H2O2 on motility and increase on the percentages of positive cells for CellROX green, capacitated sperm, and AO. A decrease on cleavage and blastocyst rates was observed as H2O2 increased. Also, we detected a blockage on embryo development. We concluded that sperm when exposed to oxidative environment presents impaired motility traits, p...Continue Reading

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Citations

Mar 17, 2016·Systems Biology in Reproductive Medicine·Gayatri MohantyLuna Samanta
Sep 23, 2016·Molecular Reproduction and Development·Yun-Wei PangHua-Bin Zhu
Jul 11, 2018·PloS One·Adriano Felipe Perez SiqueiraMayra Elena Ortiz D'Ávila Assumpção
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Feb 12, 2019·PloS One·Georges RaadMira Hazzouri
Mar 6, 2019·Zygote : the Biology of Gametes and Early Embryos·Georges RaadJoseph Azoury
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May 28, 2020·Andrologia·Cătălina BarbăroșieRalf Henkel
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Oct 14, 2020·Zygote : the Biology of Gametes and Early Embryos·Jaromír VašíčekPeter Chrenek
Dec 11, 2020·Theriogenology·Suzane Peres CampanholiLindsay Unno Gimenes
Mar 17, 2021·Reproduction in Domestic Animals = Zuchthygiene·Gabriela Bertaiolli ZocaRubens Paes de Arruda
Apr 6, 2021·Zygote : the Biology of Gametes and Early Embryos·Vivian Cardoso CastiglioniMayra Elena Ortiz D Ávila Assumpção
Feb 9, 2021·The Journal of Reproduction and Development·Tatsuya NakanoTasuku Mitani
Apr 20, 2021·Heliyon·Juan Sebastian Naranjo-GómezIang Schroniltgen Rondón-Barragán
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Oct 10, 2021·Reproduction in Domestic Animals = Zuchthygiene·Diogo Ribeiro CâmaraHeinrich Bollwein

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Methods Mentioned

BETA
flow cytometry
FCS
Assay

Software Mentioned

CellROX
FlowJo
Statistical Analysis System
Computer Assisted Sperm Analysis system ( CASA IVOS

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