Sperm pre-incubation prior to insemination affects the sex ratio of bovine embryos produced in vitro

Reproduction in Domestic Animals = Zuchthygiene
Dorota LechniakA W King

Abstract

The objective of the present study was to determine whether sperm incubation prior to oocyte insemination in vitro affects the sex ratio of resulting blastocyst. Cumulus-oocyte-complexes (COCs) collected from slaughterhouse ovaries were matured in vitro and inseminated with frozen-thawed semen of three proven artificial insemination (AI) bulls pre-incubated in vitro in Sperm-Talp for 6 and 24 h. On day-9 blastocysts were collected and processed for sex determination. More than 80% of blastocyst were successfully sexed. There were no significant differences in cleavage and blastocyst rates using sperm pre-incubated for 6 h as compared with the 0-h pre-incubation control group. The cleavage and blastocyst rates were significantly lower in the 24-h pre-incubation group. The male to female ratio, when compared with the theoretical 1 : 1, differed significantly in favour of females among hatched (viable) blastocysts derived from sperm pre-incubated for 24 h prior to insemination as well as among all blastocytsts in the 6-h group. Moreover, when the sperm treatment was considered, the sex ratio was affected only among hatched blastocysts in 24-h pre-incubation group. It was concluded that prolonged sperm pre-incubation influences the...Continue Reading

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Citations

Jan 28, 2009·Theriogenology·Katherine E M Hendricks, Peter J Hansen
Nov 11, 2015·Animal Reproduction Science·M A SilvestreJ L Yániz
Oct 23, 2010·Reproduction in Domestic Animals = Zuchthygiene·R ZobelS Tkalcic
Jan 17, 2014·Reproduction in Domestic Animals = Zuchthygiene·A AzizeddinT Revay
Aug 8, 2013·Reproduction in Domestic Animals = Zuchthygiene·M OrsztynowiczD Lechniak
Jul 10, 2009·Biology of Reproduction·Darin HylanRobert A Godke
Feb 11, 2020·Frontiers in Cell and Developmental Biology·Md Saidur Rahman, Myung-Geol Pang
Jan 19, 2013·Reproduction, Fertility, and Development·Mohammad Bozlur RahmanAnn Van Soom

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