Sperm quantity and size variation in un-irradiated and irradiated males of the malaria mosquito Anopheles arabiensis Patton

Acta Tropica
Michelle E H Helinski, Bart G J Knols

Abstract

Anopheles mosquitoes are important candidates for genetic control strategies. However, little is known about sperm quality and quantity as determinants of male reproductive success. In this study, sperm quantity and length variation were assessed in testes of un-irradiated and irradiated Anopheles arabiensis. Male reproductive organs were dissected for sperm and an estimate of the total number of spermatozoa was made. Sperm lengths were measured using imaging software. The effects of irradiation were evaluated for males exposed in the pupal or adult stage to a full (120 Gy) or partially sterilising dose (70 Gy). Sperm length variation in the laboratory strain was compared to the distribution observed in wild males. We also determined the size distribution of sperm lengths in spermathecae of inseminated females compared to those observed in male testes. Sperm quantity increased with age, and 12-day-old males had significantly more sperm in their testes (8214+/-467) than males aged 3 days (5022+/-375). Mosquitoes irradiated in the pupal stage had significantly fewer sperm (2982+/-125) than un-irradiated males (4950+/-848) although for adult stage irradiation similar amounts of sperm were observed compared to un-irradiated males. ...Continue Reading

Citations

Oct 9, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·R H HuntM Fettene
Jun 12, 2002·Biological Reviews of the Cambridge Philosophical Society·John G Swallow, Gerald S Wilkinson
Oct 5, 2002·Science·Thomas W ScottChristophe Boëte
Oct 5, 2002·Science·Luke AlpheyFrank H Collins
Jan 27, 2005·Journal of Insect Physiology·Marc J Klowden, Gail M Chambers
Oct 26, 2005·Nature Biotechnology·Flaminia CatterucciaAndrea Crisanti
May 17, 2006·Trends in Ecology & Evolution·Heather M FergusonBart G J Knols

Related Concepts

Science of Morphology
Tooth Crowding
Sperm Cell
Spermatozoa Cell Count
Drosophila
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Larva
Malaria
Malaria Vaccines
Centropomus undecimalis

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