Sperm rescue in wild African elephants

Reproduction, Fertility, and Development
J SaragustyR Hermes

Abstract

This study investigated ways of improving the usefulness of ~1700mL of poor-quality frozen semen collected from wild African elephant (Loxodonta africana) bulls. Ten semen samples from six bulls, frozen with 5% glycerol in Berliner cryomedium, with or without prior removal of the seminal plasma by centrifugation, were tested. All samples were subjected to the following density-gradient centrifugation treatments: no centrifugation (control), sham centrifugation, Percoll, OptiPrep, Isolate and PureSperm. Sample evaluation included motility, concentration, viability, acrosome integrity and normal morphology after thawing and after gradient centrifugation. Motility was also evaluated 3h after thawing. While all treatments were similar to the Control in acrosome integrity and normal morphology, significant differences were noted in concentration, viability and motility. Samples treated by Percoll showed the best motility, which was maintained unchanged over 3h of incubation (37°C). Correlations between manual and automated evaluations of concentration were high (cytometer; rho=0.92), but were lower for viability (cytometer; rho=0.57) and motility (computer-aided sperm analysis; rho=0.66). By performing density centrifugation, the qu...Continue Reading

References

Oct 13, 2004·Journal of Andrology·Nathalie Mousset-SiméonBertrand Mace
Mar 28, 2006·Theriogenology·Budhan PukazhenthiDavid E Wildt
Aug 20, 2008·Fertility and Sterility·Yogesh Kumar JaiswalMadan Mohan Chaturvedi
Dec 30, 2008·Animal Reproduction Science·Joseph SaragustyRobert Hermes
Jul 7, 2011·PloS One·Philippe BouchéCédric Vermeulen
Nov 9, 2011·Journal of Animal Science·M L W J BroekhuijseB M Gadella
Mar 14, 2013·PloS One·Robert HermesThomas B Hildebrandt

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Citations

Feb 18, 2016·Animal Reproduction Science·Amir Arav, Joseph Saragusty
Aug 27, 2016·Reproduction in Domestic Animals = Zuchthygiene·O García-ÁlvarezJ J Garde
Feb 9, 2018·Zoological Science·Sebastian J CharltonJulie Strand

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Methods Mentioned

BETA
density-gradient centrifugation
flow cytometry

Software Mentioned

Percoll
NucleoCounter
CASA
OptiPrep
Coin
R
R Core Team
SpermVision
PureSperm
nparLD

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