Sperm selected by both birefringence and motile sperm organelle morphology examination have reduced deoxyribonucleic acid fragmentation

Fertility and Sterility
Andrea GarollaCarlo Foresta

Abstract

To evaluate DNA fragmentation in single sperm selected by both birefringence and motile sperm organelle morphology examination (MSOME) with a single instrument. Prospective study. University setting. Semen samples from 33 normozoospermic subjects. Birefringence and MSOME to distinguish different categories of sperm: nonbirefringent (category A), birefringent (category B), birefringent with nuclear vacuoles (category C), and birefringent with no nuclear vacuoles (category D). From each semen sample, sperm of any category were selected and further analyzed by TUNEL test. A total of 660 well-characterized sperm were evaluated for DNA fragmentation. Category A showed a low percentage of sperm with normal MSOME results (19.4%) and high prevalence of DNA fragmentation (70.3%). Category B had 81.8% normal MSOME results, and in this group 31.8% had fragmentated DNA. Category C showed 31.8% and 92.6% DNA fragmentation in sperm with small and large nuclear vacuoles, respectively. Birefringent sperm with normal MSOME results and no vacuoles showed the lowest percentage of fragmented DNA (2.8%). Sperm selection by birefringence or MSOME alone had one-third probability to select sperm with fragmented DNA. The lowest percentage of DNA fragme...Continue Reading

References

Feb 5, 1997·Journal of the National Cancer Institute·B T JiF Jin
May 26, 1999·Journal of Reproduction and Fertility·R J Aitken
Jan 17, 2002·The New England Journal of Medicine·D S GuzickUNKNOWN National Cooperative Reproductive Medicine Network
Dec 6, 2003·Reproductive Biomedicine Online·Denny SakkasGian Carlo Manicardi
May 25, 2005·International Journal of Andrology·R J AitkenB V King
Oct 11, 2005·Fertility and Sterility·Ashok Agarwal, Shyam S R Allamaneni
Feb 4, 2006·Reproductive Biomedicine Online·André HazoutJan Tesarik
Feb 25, 2006·Human Reproduction·Arie BerkovitzBenjamin Bartoov
May 19, 2007·Reproductive Biomedicine Online·Gabor HuszarSinan Ozkavukcu
Jun 21, 2007·Reproductive Biomedicine Online·R John Aitken, Geoffry N De Iuliis
Jan 18, 2008·Biology of Reproduction·Raúl Fernández-GonzalezAlfonso Gutiérrez-Adán
Nov 6, 2008·Reproductive Biomedicine Online·Andrea GarollaCarlo Foresta
Apr 22, 2011·Reproductive Biomedicine Online·C G PetersenJ G Franco
Dec 6, 2011·Asian Journal of Andrology·Lara TamburrinoMonica Muratori
Nov 8, 2012·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Alaa HamadaAshok Agarwal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Reproductive Biology and Endocrinology : RB&E
Liliane F I SilvaJosé Gonçalves Franco
International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition
Barbara WirleitnerNicolas Herbert Zech
Reproductive Biology and Endocrinology : RB&E
Joao Batista Alcantara OliveiraJosé Gonçalves Franco
© 2021 Meta ULC. All rights reserved