Sperm subpopulations in Iberian red deer epididymal sperm and their changes through the cryopreservation process

Biology of Reproduction
F Martínez-PastorP De Paz

Abstract

We have applied a statistical protocol based on principal component analysis, clustering methods, and discriminant analysis for the identification of sperm subpopulations in computer-assisted sperm analysis (CASA) data. Samples were obtained from the cauda epididymis of 11 Iberian red deer and cryopreserved following a standard protocol. Motility by CASA was analyzed just after sperm recovery, just before freezing, and after thawing, and eight motility descriptors for each individual spermatozoon were recorded. Sperm viability and acrosomal status were also assessed. Subpopulation analysis was performed in four sequential steps: principal component analysis using the eight motility descriptors; nonhierarchical clustering analysis (k-means) using the first two principal components; hierarchical clustering analysis (UPGMA); and selection of the final number of clusters. Three clusters were obtained for each motility analysis: slow and nonlinear; rapid and linear; and rapid, high ALH, nonlinear. We detected variations in the clusters between treatments (initial, prefreezing and postthawed). Indeed, motility increased and linearity decreased in the prefreezing analysis. A discriminant analysis isolated three descriptors that were u...Continue Reading

References

Feb 1, 1991·Molecular Reproduction and Development·N OkamuraJ L Dacheux
Jan 1, 1996·Reproduction, Fertility, and Development·R A Harrison
Sep 1, 1996·Journal of Reproduction and Fertility·C G GravanceP J Casey
Jul 14, 2001·Reproduction, Fertility, and Development·A M Petrunkina, E Töpfer-Petersen
Feb 13, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·R Gualtieri, R Talevi
Mar 14, 2003·Journal of Andrology·Uwe PaaschHans-Juergen Glander
Sep 19, 2003·Acta histochemica·Daniela MeggiolaroBruno Ferrandi

❮ Previous
Next ❯

Citations

Mar 28, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Felipe Martínez-PastorMaria Teresa Dinis
Jul 4, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Alberto ContriAugusto Carluccio
Feb 12, 2013·Systems Biology in Reproductive Medicine·Luis Becerril MartínezAndrés Aragón Martínez
Jan 22, 2013·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·L Maree, G van der Horst
Nov 30, 2010·Cryobiology·J BeirãoM P Herráez
May 14, 2010·Animal Reproduction Science·J DoradoM Hidalgo
Sep 8, 2009·Theriogenology·A E Domínguez-RebolledoF Martínez-Pastor
Jul 14, 2007·Animal Reproduction Science·Manuel HidalgoCarles Soler
Sep 1, 2015·Theriogenology·A Maroto-MoralesJ J Garde
Sep 17, 2009·Reproduction in Domestic Animals = Zuchthygiene·K ChatdarongA Sangwornrachasup
Sep 21, 2006·Reproduction in Domestic Animals = Zuchthygiene·I Núñez-MartínezF J Peña
Sep 21, 2006·Reproduction in Domestic Animals = Zuchthygiene·F J PeñaJ M Morán
Sep 21, 2006·Reproduction in Domestic Animals = Zuchthygiene·L AnelP de Paz
Sep 21, 2006·Reproduction in Domestic Animals = Zuchthygiene·J J GardeE R S Roldán
Nov 11, 2008·Reproduction in Domestic Animals = Zuchthygiene·M C EstesoJ J Garde
Jan 3, 2013·Reproduction in Domestic Animals = Zuchthygiene·S KrishnakumarJ Thundathil
Oct 4, 2014·Reproduction in Domestic Animals = Zuchthygiene·M RamónJ J Garde
Jul 16, 2010·Reproduction in Domestic Animals = Zuchthygiene·F Martínez-PastorP de Paz
Jun 23, 2006·Theriogenology·Felipe Martínez-PastorPaulino de Paz

❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient centrifugation
PCA

Software Mentioned

SPSS
R
STAT
Excel
PATN
CLUSTER
PRIN2
CASA
Sperm Class Analyzer
SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.