In the adult male, a population of diploid stem-cell spermatogonia continuously undergoes self-renewal and produces progeny cells, which initiate the complex process of cellular differentiation that results in mature spermatozoa. We report here that stem cells isolated from testes of donor male mice will repopulate sterile testes when injected into seminiferous tubules. Donor cell spermatogenesis in recipient testes showed normal morpholigical characteristics and produced mature spermatozoa. This methodology, besides opening new avenues of basic research into spermatogenesis and stem-cell self-renewal, may prove useful as a tool for biomedical science and biotechnology.
Spermatogenetic clones developing from repopulating stem cells surviving a high dose of an alkylating agent
The dopamine beta-hydroxylase gene promoter directs expression of E. coli lacZ to sympathetic and other neurons in adult transgenic mice
Effects of busulfan on murine spermatogenesis: cytotoxicity, sterility, sperm abnormalities, and dominant lethal mutations
Role of spermatogonia in the repair of the seminiferous epithelium following x-irradiation of the rat testis
The spermatogonial stem cell population in adult rats. I. Their morphology, proliferation and maturation
Re-examination of spermatogonial renewal in the rat by means of seminiferous tubules mounted "in toto"
Developmentally regulated expression of a mouse germ cell nuclear antigen examined from embryonic day 11 to adult in male and female mice
Computer assisted image analysis to assess colonization of recipient seminiferous tubules by spermatogonial stem cells from transgenic donor mice
Bcl-2 inhibits apoptosis of spermatogonia and growth of spermatogonial stem cells in a cell-intrinsic manner
Germ cell development in the descended and cryptorchid testis and the effects of hormonal manipulation
Characterization of germ cells from pre-pubertal bull calves in preparation for germ cell transplantation
Colonic subepithelial myofibroblasts in mucosal inflammation and repair: contribution of bone marrow-derived stem cells to the gut regenerative response
An overview of functional and stereological evaluation of spermatogenesis and germ cell transplantation in fish
Achieving high survival rate following cryopreservation after isolation of prepubertal mouse spermatogonial cells
In vitro culture and morphological characterization of prepubertal buffalo (Bubalus bubalis) putative spermatogonial stem cell
Availability of subfertile transgenic rats expressing the c-myc gene as recipients for spermatogonial transplantation
Potentiation of testicular cytotoxicity by the alkyltransferase inhibitor O6 benzylguanine and the 5-fluorouracil/N-(2-chloroethyl)-N-nitrosourea molecular combination, B.4152
Isolation of germ cells from human testicular tissue for low temperature storage and autotransplantation
A review of in vitro gamete maturation and embryo culture and potential impact on future animal biotechnology
Transgenic mouse models and germ cell transplantation: two excellent tools for the analysis of genes regulating male fertility
Mice spermatogonial stem cells transplantation induces macrophage migration into the seminiferous epithelium and lipid body formation: high-resolution light microscopy and ultrastructural studies
Can we grow sperm? A translational perspective on the current animal and human spermatogenesis models
Adult Stem Cells
Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.
Bioinformatics in Biomedicine
Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.