PMID: 874003Jul 1, 1977

Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization

The Journal of Cell Biology
A R BellvéM Dym

Abstract

A procedure is described which permits the isolation from the prepuberal mouse testis of highly purified populations of primitive type A spermatogonia, type A spermatogonia, type B spermatogonia, preleptotene primary spermatocytes, leptotene and zygotene primary spermatocytes, pachytene primary spermatocytes and Sertoli cells. The successful isolation of these prepuberal cell types was accomplished by: (a) defining distinctive morphological characteristics of the cells, (b) determining the temporal appearance of spermatogenic cells during prepuberal development, (c) isolating purified seminiferous cords, after dissociation of the testis with collagenase, (d) separating the trypsin-dispersed seminiferous cells by sedimentation velocity at unit gravity, and (e) assessing the identity and purity of the isolated cell types by microscopy. The seminiferous epithelium from day 6 animals contains only primitive type A spermatogonia and Sertoli cells. Type A and type B spermatogonia are present by day 8. At day 10, meiotic prophase is initiated, with the germ cells reaching the early and late pachytene stages by 14 and 18, respectively. Secondary spermatocytes and haploid spermatids appear throughout this developmental period. The purit...Continue Reading

References

Jul 13, 1999·Cell Motility and the Cytoskeleton·M L FanarragaJ C Zabala
Apr 1, 1996·Molecular Reproduction and Development·K A CaldwellM A Handel
Sep 1, 1996·Molecular Reproduction and Development·L L TresA L Kierszenbaum
Apr 1, 1997·Molecular Reproduction and Development·J CobbM A Handel
Apr 21, 1998·Molecular Reproduction and Development·Y L ZhangD A O'Brien
Oct 3, 2000·Molecular Reproduction and Development·I DobrinskiR L Brinster
May 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S M Harrison, S Roffler-Tarlov
Dec 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K Rhee, D J Wolgemuth
Jan 1, 1994·Developmental Genetics·P MastrangeloS R Blecher
Jan 1, 1995·Developmental Genetics·M A HandelT Wiltshire
Nov 1, 1991·Molecular Reproduction and Development·A A AlcivarN B Hecht
Jan 1, 1995·Molecular Reproduction and Development·J E SiiteriD W Hamilton
Mar 1, 1995·Molecular Reproduction and Development·W GuN B Hecht
Nov 1, 1995·Molecular Reproduction and Development·A I PackerR F Bachvarova
Dec 1, 1995·Molecular Reproduction and Development·K D FulcherE M Eddy
Aug 1, 1989·Cell and Tissue Research·L M Chapman, E M Eddy
Sep 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S M KerrH J Cooke
Jun 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·P S BurkeD J Wolgemuth
Mar 1, 1994·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·K FurukawaY Hotta
May 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Y C ChoiN B Hecht
Jun 6, 2009·Cell and Tissue Research·Dorde KomljenovicKarin Gorgas
Oct 24, 2013·Ecotoxicology·Lihui WangXiaodong Han
Feb 10, 2009·Journal of Assisted Reproduction and Genetics·Firooz Jannat AlipoorMehdi Lotfi Panah
Jun 28, 2012·Journal of Assisted Reproduction and Genetics·Morteza KorujiMohammad Ali Gilani Sedighi

Citations

Jul 1, 1977·The Journal of Cell Biology·C F Millette, A R Bellvé
Mar 1, 1975·Endocrinology·M J Welsh, J P Wiebe
Jul 1, 1975·Molecular and Cellular Endocrinology·J H DorringtonI B Fritz
Jan 1, 1975·Endocrine Research Communications·A SteinbergerE Steinberger
Mar 1, 1975·Experimental Cell Research·J C Davis, A W Schuetz
Feb 1, 1975·The American Journal of Anatomy·Y CLERMONT, L Hermo
Jan 1, 1971·Zeitschrift für Zellforschung und mikroskopische Anatomie·B Gondos, C J Hobel
Nov 1, 1973·Journal of Reproduction and Fertility·E C ROOSEN-RUNGE
Oct 1, 1972·Journal of Cellular Physiology·M L Meistrich
Feb 1, 1974·Experimental Cell Research·M Loir, M Lanneau
Jan 1, 1969·Zeitschrift für Zellforschung und mikroskopische Anatomie·L Nicander, L Plöen
Mar 1, 1971·The Anatomical Record·E F OAKBERG
Jan 1, 1967·Zeitschrift für Zellforschung und mikroskopische Anatomie·L Nicander
Jun 1, 1967·The Anatomical Record·C Flickinger, D W FAWCETT
Nov 20, 1952·Annals of the New York Academy of Sciences·E C ROOSEN-RUNGE
Jul 25, 1956·The Journal of Biophysical and Biochemical Cytology·D W FAWCETT
Mar 1, 1957·The American Journal of Anatomy·Y CLERMONT, B PEREY
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT

Related Concepts

Pachytene
Centrifugation, Density Gradient
Benign Neoplasm of Testis
Cockayne Syndrome
Mitotic Prophase
Pachytene Stage
Neoplasm of Uncertain or Unknown Behavior of Testis
Cell Nucleus
Trypsin
Structure of Sertoli Cell

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