Spermidine-dependent proteins are involved in the fusion of mouse alveolar macrophages induced by 1 alpha,25-dihydroxyvitamin D3 and interleukin 4

Experimental Cell Research
H TanakaT Suda

Abstract

We have reported that 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3] directly induces fusion of mouse alveolar macrophages by a mechanism involving protein synthesis (H. Tanaka et al., 1984, FEBS Lett. 174, 61). While examining further the mechanism of the fusion, we found that polyamines, most likely spermidine, are involved as an important intracellular mediator of the 1 alpha,25(OH)2D3 action in inducing protein synthesis, which in turn induces fusion of macrophages (T. Hayashi et al., 1986, J. Bone Miner. Res. 1, 235). In this study, spermidine-dependent proteins responsible for inducing fusion were examined by electrophoresis of [35S]methionine-labeled proteins. 1 alpha,25(OH)2D3 increased synthesis of 14 proteins at 24 h after the addition, before it initiated fusion at 36 h. When spermidine synthesis was inhibited by adding methylglyoxal bis(guanylhydrazone) (MGBG), the enhanced synthesis in 9 of the 14 proteins induced by 1 alpha,25(OH)2D3 was greatly diminished with a concomitant inhibition of fusion. Further addition of spermidine restored the synthesis of these 9 proteins and the fusion as well. The synthesis of 3 of the 9 proteins was similarly induced by interferon-gamma, retinoic acid, or lipopolysaccharides, ...Continue Reading

References

Jan 1, 1976·Annual Review of Biochemistry·H F DeLuca, H K Schnoes
Apr 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T HayashiT Suda
Feb 1, 1988·The Journal of Experimental Medicine·A McInnes, D M Rennick
Jan 1, 1987·Annual Review of Immunology·W E Paul, J Ohara
Feb 1, 1988·Cell Differentiation·J L Lognonne, J P Wahrmann
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D RennickL Gemmell
Aug 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C MiyauraT Suda
Oct 1, 1986·Experimental Cell Research·J L Lognonne, J P Wahrmann
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G D RoodmanG R Mundy
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J RosenbergC Kayalar
Jul 25, 1972·Biochemical and Biophysical Research Communications·J van der BoschD Pette
Nov 1, 1969·Experimental Cell Research·A ShainbergD Yaffe
Sep 1, 1984·Journal of Cellular Physiology·D Z EwtonJ R Florini
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J B WeinbergM A Misukonis
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E AbeT Suda
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Z Bar-ShavitA J Kahn
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E AbeT Suda
Mar 1, 1984·British Journal of Cancer·K TachibanaY Kishino
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E AbeT Suda

❮ Previous
Next ❯

Citations

Dec 21, 1990·Molecular and Cellular Endocrinology·C H JinT Suda
Jun 4, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H AmanoR Felix
Sep 30, 1992·Annals of the New York Academy of Sciences·H F DeLuca

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.