Spermidine induces cytoprotective autophagy of female germline stem cells in vitro and ameliorates aging caused by oxidative stress through upregulated sequestosome-1/p62 expression.

Cell & Bioscience
Xiaoyan YuanJi Wu

Abstract

Autophagy is required for oogenesis and plays a critical role in response to aging caused by oxidative stress. However, there have been no reports on regulation of cytoprotective autophagy in female germline stem cells (FGSCs) in response to aging caused by oxidative stress. We found that Spermidine (SPD) significantly increased protein expression of autophagy markers microtubule-associated protein 1 light chain 3 beta-II (MAP1LC3B-II/LC3B-II) and sequestosome-1/p62 (SQSTM1/p62), and evoked autophagic flux in FGSCs. Moreover, SPD increased the number and viability of FGSCs in vitro. Further, we found that SPD significantly reduced basal or hydrogen peroxide (H2O2)-induced up-regulated protein expression of the aging markers, cyclin dependent kinase inhibitor 2A (p16/CDKN2A) and tumor protein 53 (p53). After knockdown of p62 in FGSCs, p16 protein levels were significant higher compared with controls. However, protein p16 levels were not significantly changed in p62 knockdown FGSCs with SPD treatment compared with without SPD. Moreover, SPD significantly changed the expression of autophagy-related genes and pathways in FGSCs, as shown by bioinformatics analysis of RNA sequencing data. Additionally, SPD significantly inhibited AKT...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 17, 2008·Cell Death and Differentiation·T Vellai
Sep 7, 2012·Rejuvenation Research·Stefania PucciarelliValerio Napolioni
Jan 8, 2016·Nature·Laura García-PratPura Muñoz-Cánoves
Feb 27, 2016·Molecular Human Reproduction·Kun GuoPing Zheng
May 14, 2016·Circulation Research·Akihiro ShirakabeJunichi Sadoshima
Nov 15, 2016·Nature Medicine·Tobias EisenbergFrank Madeo
Jan 25, 2017·Autophagy·Tobias EisenbergFrank Madeo
Jun 3, 2017·Cell·Shenghui He, Norman E Sharpless
Jan 27, 2018·Science·Frank MadeoGuido Kroemer
May 31, 2019·Human Genetics·Shi Q WongLouis R Lapierre
Aug 10, 2019·Frontiers in Cell and Developmental Biology·Tamás MaruzsGábor Juhász
Aug 20, 2019·Ecotoxicology and Environmental Safety·Dan SongRong Liu
Sep 6, 2019·Animal Science Journal = Nihon Chikusan Gakkaihō·Xuerui YaoNam-Hyung Kim
Oct 19, 2019·Nature Communications·Miguel A Muñoz-LorenteMaria A Blasco
Nov 2, 2019·Cell·Vassilis GorgoulisMarco Demaria
Jan 26, 2020·Aging Cell·Matthew J YousefzadehLaura J Niedernhofer
Oct 3, 2020·Antioxidants·Nimmy VargheseAnne Eckert

❮ Previous
Next ❯

Software Mentioned

Pro Plus
Image
GraphPad Prism
R

Related Concepts

Related Feeds

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Cell Aging

This feed focuses on cellular aging with emphasis on mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms