Spermidine synthase genes are essential for survival of Arabidopsis

Plant Physiology
Akihiro ImaiTaku Takahashi

Abstract

The cellular polyamines putrescine, spermidine, and spermine are ubiquitous in nature and have been implicated in a wide range of growth and developmental processes. There is little information, however, on mutant plants or animals defective in the synthesis of polyamines. The Arabidopsis genome has two genes encoding spermidine synthase, SPDS1 and SPDS2. In this paper, we describe T-DNA insertion mutants of both of these genes. While each mutant allele shows normal growth, spds1-1 spds2-1 double-mutant seeds are abnormally shrunken and they have embryos that are arrested morphologically at the heart-torpedo transition stage. These seeds contain significantly reduced levels of spermidine and high levels of its precursor, putrescine. The embryo lethal phenotype of spds1-1 spds2-1 is complemented by the wild-type SPDS1 gene. In addition, we observed a nearly identical seed phenotype among an F2 seed population from the cross between the spds2-1 allele and SPDS1 RNA interference transgenic lines. These data provide the first genetic evidence indicating a critical role of the spermidine synthase in plant embryo development.

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Citations

Jul 3, 2008·Planta·T KusanoY Takahashi
Mar 12, 2010·Planta·Rubén AlcázarAntonio F Tiburcio
Jul 29, 2011·Planta·Vaishali MulangiPaul Morris
Jun 20, 2012·Planta·Vaishali MulangiPaul F Morris
Oct 10, 2006·Biotechnology Letters·Rubén AlcázarTeresa Altabella
Feb 1, 2012·Journal of Experimental Botany·Julia Krasensky, Claudia Jonak
Jul 26, 2008·Molecular Biology and Evolution·Eugenio G MinguetMiguel A Blázquez
Aug 2, 2008·Plant & Cell Physiology·Jun-ichi KakehiTaku Takahashi
Aug 16, 2008·Plant & Cell Physiology·Tomoe Kamada-NobusadaMikio Nishimura
Mar 1, 2012·Plant & Cell Physiology·Ayaka TakanoTaku Takahashi
May 2, 2006·Plant Physiology·Willem Van de VeldeSofie Goormachtig
Oct 18, 2005·BMC Structural Biology·Piotr Z Kozbial, Arcady R Mushegian
Nov 13, 2013·Journal of Experimental Botany·Panagiotis N Moschou, Kalliopi A Roubelakis-Angelakis
Mar 25, 2014·Planta·Antonio F TiburcioRubén Alcázar
Apr 1, 2014·Molecular Plant-microbe Interactions : MPMI·Shan-Shan XieXue-Wen Gao
Dec 5, 2006·FEBS Letters·Koji YamaguchiTomonobu Kusano
Jan 17, 2016·Plant Physiology and Biochemistry : PPB·Georgios TsaniklidisCostas Delis
Mar 27, 2012·Phytochemistry·Yvonne Sichhart, Birgit Dräger
Jul 21, 2010·Journal of Plant Physiology·Maria C Gomez-JimenezIsabel M Sanchez-Calle
Apr 13, 2010·Plant Physiology and Biochemistry : PPB·Margarita Rodríguez-KesslerJuan Francisco Jiménez-Bremont
Mar 17, 2010·Plant Physiology and Biochemistry : PPB·Christine FuellAnthony J Michael

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