Spermine as a porcine pancreatic elastase activator: spectroscopic and molecular simulation studies

Journal of Biomolecular Structure & Dynamics
Sakineh Sadeghi-KajiSadegh Farhadian

Abstract

The aim of this study was to investigate the spermine effect on the thermal denaturation, conformation and activity of elastase at three temperatures of 303, 313 and 323 K in the Tris buffer, at pH 8.5, using UV-vis spectrophotometry, spectrofluorometry and circular dichroism as well as molecular docking and molecular simulation. The increased absorption of elastase in the presence of spermine suggested a change in the environment of tryptophan. It was found that under the influence of spermine, the emission intensity of elastase extremely was reduced, and the use of the Stern-Volmer equation showed that some static quenching had occurred. The thermodynamic parameters values (enthalpy and entropy) and the molecular docking technique also revealed that van der Waals forces or hydrogen bonding interactions played an important role in the binding process. The spermine-elastase complex formation led to increasing the value of the catalytic constant (kcat). So it could be considered as an activator. Slight changes were observed in the second structure of elastase (1.06% increase for the α-helix and 0.048% decrease the β-sheet) and the thermal stability effect. Molecular docking results also demonstrated that spermine could bind to p...Continue Reading

References

Mar 31, 2000·Acta Crystallographica. Section D, Biological Crystallography·M WürteleW Höhne
Apr 18, 2002·Current Opinion in Structural Biology·Tomas HanssonWilfredF van Gunsteren
Aug 4, 2004·Amino Acids·U Bachrach
Oct 6, 2005·Journal of Cellular and Molecular Medicine·Nikolaus Seiler, Francis Raul
Dec 31, 2005·Current Protein & Peptide Science·Uriel Bachrach
Sep 14, 2007·International Journal of Biological Macromolecules·Nasrollah Rezaei-GhalehMohsen Nemat-Gorgani
Dec 17, 2008·Connective Tissue Research·Deepa NadarajahBarry Starcher
Mar 17, 2010·Journal of Molecular Graphics & Modelling·Shao-Yong LuWei-Liang Zhu
Aug 5, 2010·Biomacromolecules·Zhenxing ChiHao Zhang
Jul 10, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ying Liu, Rutao Liu
Mar 5, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xinxin HuRutao Liu
Jul 9, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hua LiRuiqiang Wang
Jul 9, 2013·Luminescence : the Journal of Biological and Chemical Luminescence·Maryam Mahmoodian MoghaddamJamshidkhan Chamani
Oct 29, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Behafarid GhalandariMassoud Amanlou
Jul 29, 2015·The Journal of Physical Chemistry. B·Hassan MonhemiAli Nakhaei Pour
Aug 19, 2015·Physical Chemistry Chemical Physics : PCCP·Trung Quan Luong, Roland Winter
Nov 1, 2005·Journal of Chemical Theory and Computation·Elena PapaleoLuca De Gioia
Jan 17, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·A SudhaN Sengottuvelan
Mar 1, 2016·Journal of Biomolecular Structure & Dynamics·Sadegh FarhadianAli A Saboury
Apr 19, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mingyang JingRutao Liu
May 24, 2016·Journal of Biomolecular Structure & Dynamics·Lida MomeniAli Akbar Saboury
Oct 30, 2016·International Journal of Biological Macromolecules·Lida MomeniFateme Reisi
Oct 30, 2016·International Journal of Biological Macromolecules·Mansoore Hosseini-KoupaeiFateme Davar
Jul 31, 2018·International Journal of Biological Macromolecules·Lida MomeniFateme Raisi
Nov 9, 2018·International Journal of Biological Macromolecules·Mansoore Hosseini-KoupaeiZahra Enteshari

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Methods Mentioned

BETA
circular dichroism
chemical modification

Software Mentioned

DSSP
Ligplot
plot
Lig plot +
AutoDock
Lig
Mercury
CDNN

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