Spermiogenesis deficiency and germ-cell apoptosis in CREM-mutant mice

Nature
F NantelP Sassone-Corsi

Abstract

Spermiogenesis is a complex process by which postmeiotic male germ cells differentiate into mature spermatozoa. This process involves remarkable structural and biochemical changes including nuclear DNA compaction and acrosome formation. Transcription activator CREM (cyclic AMP-responsive element modulator) is highly expressed in postmeiotic cells, and CREM may be responsible for the activation of several haploid germ cell-specific genes involved in the structuring of the spermatozoon. The specific role of CREM in spermiogenesis was addressed using CREM-mutant mice generated by homologous recombination. Analysis of the seminiferous epithelium in mutant male mice reveals postmeiotic arrest at the first step of spermiogenesis. Late spermatids are completely absent, and there is a significant increase in apoptotic germ cells. We show that CREM deficiency results in the lack of postmeiotic cell-specific gene expression. The complete lack of spermatozoa in the mutant mice is reminiscent of cases of human infertility.

References

Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·M R Rubin, M C Nguyen-Huu
Feb 7, 1992·Cell·N S Foulkes, P Sassone-Corsi
Jan 29, 1985·Biochemistry·K C KleeneN B Hecht
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P LemaireP Charnay
May 6, 1988·Biochimica Et Biophysica Acta·P A JohnsonN B Hecht
Jul 13, 1988·Biochimica Et Biophysica Acta·K C KleeneN B Hecht
Jan 1, 1982·Endocrine Reviews·M Parvinen
Oct 28, 1993·Nature·J C ChriviaR H Goodman

❮ Previous
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Citations

Jan 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C MoriE M Eddy
Feb 9, 1999·Prenatal Diagnosis·E D KimD J Lamb
Jan 1, 1997·Journal of Cellular Biochemistry·H HaN B Hecht
Jul 21, 1998·Molecular Reproduction and Development·G C HummelkeA J Cooney
Mar 29, 2000·Molecular Reproduction and Development·M L SinclairL R Levin
May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C G Print, K L Loveland
Sep 23, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D J Elliott, H J Cooke
Aug 14, 2001·Journal of Cellular Biochemistry·H J SchultenP Burfeind
Jan 10, 2002·Journal of Biosciences·U Natraj
Jul 11, 2008·Molecular Genetics and Genomics : MGG·Claudia LalancetteStephen A Krawetz
Aug 1, 1996·Trends in Genetics : TIG·A V Anagnostopoulos
May 1, 1996·Trends in Endocrinology and Metabolism : TEM·W H Walker, J F Habener
Dec 5, 2003·Molecular and Cellular Endocrinology·Tim BeissbarthGünther Schütz
Apr 28, 2000·Fertility and Sterility·S J SilberA Van Steirteghem
Oct 23, 1997·The Journal of Urology·M Goldstein
Jul 12, 1996·Cell·R Aramayo, R L Metzenberg
Nov 5, 1997·Trends in Neurosciences·N S FoulkesP Sassone-Corsi
May 2, 1997·Biochimica Et Biophysica Acta·M KapelleW D Schleuning
Nov 7, 1998·Biochimica Et Biophysica Acta·R Adroer, R Oliva
Jun 10, 1998·Biology of the Cell·N S FoulkesP Sassone-Corsi
Jun 23, 2001·Molecular and Cellular Endocrinology·G M FimiaP Sassone-Corsi
May 4, 2002·Molecular and Cellular Endocrinology·D RozmanR Frangez
May 4, 2002·Molecular and Cellular Endocrinology·Jeremy Don, Gil Stelzer
Jan 28, 1999·Molecular and Cellular Endocrinology·J A GrootegoedJ H Hoeijmakers
Dec 9, 1996·Biochimica Et Biophysica Acta·N S Foulkes, P Sassone-Corsi
Jul 3, 1999·Trends in Biochemical Sciences·D De CesareP Sassone-Corsi
Jul 1, 1997·Trends in Endocrinology and Metabolism : TEM·C R EstherK E Bernstein
Jul 27, 2001·The International Journal of Biochemistry & Cell Biology·V Budhram-MahadeoD S Latchman
May 23, 1998·The International Journal of Biochemistry & Cell Biology·P Sassone-Corsi
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·B Mayr, M Montminy
Jan 5, 2011·Asian Journal of Andrology·Laszlo Nanassy, Douglas T Carrell

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