Sphere-derived tumor cells exhibit impaired metastasis by a host-mediated quiescent phenotype

Oncotarget
Anne-Marie BleauFernando Lecanda

Abstract

The spread of lung cancer cells to distant sites represents a common event associated with poor prognosis. A fraction of tumor cells named cancer stem cells (CSCs) have the ability to overcome therapeutic stress and remain quiescent. However, whether these CSCs have also the capacity to initiate and sustain metastasis remains unclear. Here, we used tumor sphere cultures (TSC) isolated from mouse and human lung cancer models to enrich for CSCs, and assessed their metastatic potential as compared to non-CSCs. As expected, TSC overexpressed a variety of stem cell markers and displayed chemoresistance. The CSC phenotype of TSC was confirmed by their higher growth ability in soft agar and tumorigenic potential in vivo, despite their reduced in vitro cell growth kinetics. Surprisingly, the appearance of spontaneous lung metastases was strongly delayed in mice injected with TSC as compared to non-TSC cells. Similarly, this finding was confirmed in several other models of metastasis, an effect associated with a retarded colonization activity. Interestingly, such delay correlated with a quiescent phenotype whose underlined mechanisms included an increase in p27 protein and lower phospho-ERK1/2 levels. Thus, these data suggest that cells...Continue Reading

References

Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Jan 2, 2004·Breast Cancer Research and Treatment·George N NaumovAnn F Chambers
Mar 12, 2004·European Journal of Nuclear Medicine and Molecular Imaging·Vladimir PonomarevJuri Gelovani Tjuvajev
Apr 2, 2005·Nature Reviews. Cancer·Michael DeanSusan Bates
Oct 26, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Robert E Coleman
Nov 24, 2006·Nature·Lucia Ricci-VitianiRuggero De Maria
Feb 7, 2007·Cancer Research·Chenwei LiDiane M Simeone
Oct 25, 2007·Nature Reviews. Cancer·Julio A Aguirre-Ghiso
Apr 3, 2008·Cancer Research·Silvestre VicentFernando Lecanda
Sep 8, 2009·Nature Medicine·Piyush B GuptaRobert A Weinberg
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Giulia BertoliniGabriella Sozzi
Dec 24, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Emmanuelle Charafe-JauffretMax S Wicha
Jun 16, 2010·The Journal of Investigative Dermatology·Tobias Schatton, Markus H Frank
Jan 20, 2011·Nature Communications·Vinagolu K RajasekharHoward I Scher
May 5, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mariano Ponz-SarviséIgnacio Gil-Bazo
Nov 10, 2011·Assay and Drug Development Technologies·Elizabeth TranYe Fang
Nov 30, 2011·The Journal of Experimental Medicine·Aya KobayashiKounosuke Watabe
Jan 13, 2012·Nature Reviews. Cancer·Long V NguyenConnie J Eaves
Apr 17, 2012·The Journal of Clinical Investigation·Toni Celià-TerrassaTimothy M Thomson
May 2, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ines Martin-PaduraAlfonso Calvo
May 5, 2012·Seminars in Cancer Biology·Christina Scheel, Robert A Weinberg
May 23, 2012·Cancer Discovery·Tsukasa ShibueRobert A Weinberg
Aug 23, 2012·PloS One·Devin T RosenthalSofia D Merajver
Oct 9, 2012·International Journal of Oncology·Christian LehmannManfred Kubbies

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Citations

Apr 14, 2016·Annals of Surgical Treatment and Research·Eunkyung ChungKil Yeon Lee
Jan 31, 2021·Nature Communications·Catherine A VaughanSumitra Deb

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Methods Mentioned

BETA
flow cytometry
X-Ray
xenograft
PCR
bioluminescence imaging

Software Mentioned

Cell Quest Pro
ImageJ
Imaging System
Living Image
AnalySIS
FlowJo

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