Sphere-Formation Assay: Three-Dimensional in vitro Culturing of Prostate Cancer Stem/Progenitor Sphere-Forming Cells

Frontiers in Oncology
Hisham F BahmadWassim Abou-Kheir

Abstract

Cancer Stem Cells (CSCs) are a sub-population of cells, identified in most tumors, responsible for the initiation, recurrence, metastatic potential, and resistance of different malignancies. In prostate cancer (PCa), CSCs were identified and thought to be responsible for the generation of the lethal subtype, commonly known as Castration-Resistant Prostate Cancer (CRPC). In vitro models to investigate the properties of CSCs in PCa are highly required. Sphere-formation assay is an in vitro method commonly used to identify CSCs and study their properties. Here, we report the detailed methodology on how to generate and propagate spheres from PCa cell lines and from murine prostate tissue. This model is based on the ability of stem cells to grow in non-adherent serum-free gel matrix. We also describe how to use these spheres in histological and immuno-fluorescent staining assays to assess the differentiation potential of the CSCs. Our results show the sphere-formation Assay (SFA) as a reliable in vitro assay to assess the presence and self-renewal ability of CSCs in different PCa models. This platform presents a useful tool to evaluate the effect of conventional or novel agents on the initiation and self-renewing properties of diffe...Continue Reading

Citations

May 11, 2019·PloS One·Michael E BregenzerGeeta Mehta
Jun 9, 2019·Molecular Pharmacology·Kay Yi ChongYang Yang-Hartwich
Sep 6, 2019·Journal of Cancer Research and Clinical Oncology·Hirofumi OmoriMuneyuki Masuda
Dec 5, 2019·Drug Development Research·Komal N PadhariyaPrashant S Kharkar
Aug 13, 2020·Drug Development Research·Komal N PadhariyaPrashant S Kharkar
Jun 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nada Al MatariRami Mhanna
Jun 10, 2020·Cell Death & Disease·Nabanita MukherjeeYiqun G Shellman
Apr 4, 2019·Frontiers in Oncology·Larry BodgiWassim Abou-Kheir
Oct 24, 2020·Drug Delivery and Translational Research·Jin CaoJayanth Panyam
Oct 31, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tzu-Cheng SungQingsong Ye
Nov 17, 2020·Cancer Biology & Therapy·Hiromi KonishiMasahide Ohmichi
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Katia CheaitoWassim Abou-Kheir
Dec 19, 2020·Frontiers in Molecular Biosciences·Catarina I G PintoFilipa Mendes
Dec 17, 2020·International Journal of Molecular Sciences·Xingshuo ZhangBenjamin Gantenbein
Jan 23, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Deepak RajJohn F Marshall
May 1, 2021·International Journal of Molecular Sciences·Brock HumphriesChengfeng Yang
Aug 18, 2021·Technology in Cancer Research & Treatment·Ameneh JafariHakimeh Zali

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Methods Mentioned

BETA
Dissection
mechanical dissociation
light microscopy
confocal microscopy
Xenografts
biopsies

Software Mentioned

PrEGM BulletKit
Zeiss ZEN

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