Sphere-forming culture enriches liver cancer stem cells and reveals Stearoyl-CoA desaturase 1 as a potential therapeutic target

BMC Cancer
Xiao-Lu MaXin-Rong Yang

Abstract

The role of sphere-forming culture in enriching subpopulations with stem-cell properties in hepatocellular carcinoma (HCC) is unclear. The present study investigates its value in enriching cancer stem cells (CSCs) subpopulations and the mechanism by which HCC CSCs are maintained. HCC cell lines and fresh primary tumor cells were cultured in serum-free and ultra-low attachment conditions to allow formation of HCC spheres. In vitro and in vivo experiments were performed to evaluate CSC characteristics. Expression levels of CSC-related genes were assessed by qRT-PCR and the correlation between sphere formation and clinical characteristics was investigated. Finally, gene expression profiling was performed to explore the molecular mechanism underlying HCC CSC maintenance. We found that both cell lines and primary tumor cells formed spheres. HCC spheres possessed the capacity for self-renewal, proliferation, drug resistance, and contained different subpopulations of CSCs. Of interest, 500 sphere-forming Huh7 cells or 200 primary tumor cells could generate tumors in immunodeficient animals. Sphere formation correlated with size, multiple tumors, satellite lesions, and advanced stage. Further investigation identified that the PPARα-SCD...Continue Reading

References

Jun 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tetsuhiro ChibaHideki Taniguchi
Feb 16, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Zhen Fan YangSheung Tat Fan
Aug 11, 2010·The Journal of Clinical Investigation·Naotsugu HaraguchiMasaki Mori
Mar 5, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jordi BruixUNKNOWN American Association for the Study of Liver Diseases
May 10, 2011·Cell Stem Cell·Erika PastranaFiona Doetsch
Mar 24, 2012·Cancer Cell·Jeffrey A MageeSean J Morrison
Aug 21, 2012·International Journal of Biological Sciences·Yi ChenRong-Guang Shao
Sep 21, 2013·Nature·Corbin E Meacham, Sean J Morrison
Feb 18, 2014·Gut·Jordi BruixVincenzo Mazzaferro
Feb 15, 2015·Critical Reviews in Oncology/hematology·Kuo-Shyang JengChi-Juei Jeng
Feb 18, 2016·Scientific Reports·Atsushi TakaiXin Wei Wang
Sep 17, 2016·Journal of the National Cancer Institute·Stephen B KeysarAntonio Jimeno
Sep 17, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kelli B PointerJohn S Kuo
Oct 22, 2016·Biochimica Et Biophysica Acta·R Ariel Igal
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Apr 12, 2017·Nature Reviews. Clinical Oncology·Tsukasa Shibue, Robert A Weinberg
Sep 16, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Oriana Lo ReManlio Vinciguerra
Sep 1, 2018·Epigenetics : Official Journal of the DNA Methylation Society·Oriana Lo ReManlio Vinciguerra

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Citations

Jul 17, 2020·Computational and Mathematical Methods in Medicine·Xinhua XuRongbiao Ying
Mar 15, 2020·International Journal of Molecular Sciences·Shigemi KoyamaGoshi Shiota
Jan 12, 2021·Frontiers in Cell and Developmental Biology·Se-Ra ParkHwa-Yong Lee
Apr 10, 2021·Frontiers in Oncology·Yan TanPeng Wei
May 1, 2021·International Journal of Molecular Sciences·Miriam LongoPaola Dongiovanni
Nov 2, 2020·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Qin HanHong-Yang Wang
Nov 1, 2021·Veterinary and Comparative Oncology·Koangyong SungMasahiro Okumura

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Methods Mentioned

BETA
PCR

Software Mentioned

SPSS

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