Spheroid-based 3-dimensional culture models: Gene expression and functionality in head and neck cancer

Oncology Reports
Marianne SchmidtJeanette Roller

Abstract

In the present study a panel of 12 head and neck cancer (HNSCC) cell lines were tested for spheroid formation. Since the size and morphology of spheroids is dependent on both cell adhesion and proliferation in the 3-dimensional (3D) context, morphology of HNSCC spheroids was related to expression of E-cadherin and the proliferation marker Ki67. In HNSCC cell lines the formation of tight regular spheroids was dependent on distinct E-cadherin expression levels in monolayer cultures, usually resulting in upregulation following aggregation into 3D structures. Cell lines expressing only low levels of E-cadherin in monolayers produced only loose cell clusters, frequently decreasing E-cadherin expression further upon aggregation. In these cell lines no epidermal growth factor receptor (EGFR) upregulation occurred and proliferation generally decreased in spheroids/aggregates independent of E-cadherin expression. In a second approach a global gene expression analysis of the larynx carcinoma cell line HLaC78 monolayer and the corresponding spheroids was performed. A global upregulation of gene expression in HLaC78 spheroids was related to genes involved in cell adhesion, cell junctions and cytochrome P450-mediated metabolism of xenobioti...Continue Reading

References

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Citations

Dec 12, 2017·Proteomics. Clinical Applications·Mitchell AclandPeter Hoffmann
May 10, 2019·Scientific Reports·Marloes ZoetemelkPatrycja Nowak-Sliwinska
Oct 30, 2016·Journal of Cellular Physiology·Maddaly RaviAishwarya Pattabhi
Feb 19, 2020·Current Pharmaceutical Design·Eleonore Fröhlich
Oct 16, 2019·Oncology Letters·Takahiro YoshidaTrinity J Bivalacqua
Feb 11, 2021·International Journal of Molecular Sciences·Elisabetta Colombo, Maria Grazia Cattaneo
Mar 6, 2021·Cancer Cell International·Se Jik HanKyung Sook Kim
Mar 23, 2021·Nature Communications·Tatiana Hurtado de MendozaKazuki N Sugahara
Apr 30, 2021·Acta Biomaterialia·Anna StejskalováBenjamin D Almquist
May 27, 2021·Scientific Reports·Andrea Antonio ElleroTracey Hurrell

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