Spheroplast induction in clinical isolates of Serratia marcescens in the presence of Ca2+ or Mg2+.

Journal of Clinical Microbiology
Y Tada, J Yamaguchi

Abstract

Serratia marcescens was easily induced to form spheroplasts by beta-lactam antibiotics in the presence of Ca2+ or Mg2+ without an osmotic stabilizer such as sucrose. The spheroplasts grew in volume, although they could not divide. They were stable for more than 10 h at 37 degrees C in a medium containing a high concentration of antibiotic, and they had the ability to revert to the original bacillary form. Ca2+ was more effective in spheroplast induction than Mg2+. The effect was proportional to the concentration of cations. In 40% of 180 clinical isolates of S. marcescens, more than 40% of the original bacterial cells were induced to form spheroplasts by ceftizoxime in a medium supplemented with 40 mM Ca2+. A high spheroplast induction rate was observed even in medium with 10 mM Ca2+. Few isolates that were supersusceptible to ceftizoxime (MIC, less than 0.2 microgram/ml) were induced to form spheroplasts at a high rate. No difference in spheroplast induction rate or extent between antibiotic-resistant strains and relatively susceptible strains (MIC, greater than 0.2 microgram/ml) was found. The serotype of S. marcescens had no effect on the spheroplast induction rate. Monocations (Na+ and K+) had little effect on spheroplast i...Continue Reading

References

Aug 1, 1965·Journal of General Microbiology·A W Rodwell
Dec 1, 1981·The Journal of Hospital Infection·D J Platt, J S Sommerville
May 1, 1980·The Journal of Infectious Diseases·L S TompkinsS Falkow
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Sep 1, 1964·Journal of General Microbiology·S RAZIN
Nov 1, 1964·Journal of Bacteriology·F M HAROLD

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Citations

Jan 1, 1994·Microbiology and Immunology·Y Tada, J Yamaguchi
Sep 18, 2018·Microbiology·Koki NishinoHiromi Nishida

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