Sphingolipid C4 hydroxylation influences properties of yeast detergent-insoluble glycolipid-enriched membranes

FEBS Letters
Jolanta Idkowiak-BaldysJ Y Takemoto

Abstract

Sphingoid base C4 hydroxylation is required for syringomycin E action on the yeast plasma membrane. Detergent-insoluble glycolipid-enriched membranes (DIGs) from a yeast strain lacking C4 hydroxylated sphingoid bases (sur2delta) are composed of linear membrane fragments instead of vesicular structures observed for wild-type DIGs, though they have similar lipid compositions and amounts of DIG marker proteins. Light-scattering bands collected from sur2delta after centrifugation of Triton X-100-treated cell lysates in continuous density gradients have lower buoyant densities than that of the wild-type. The results show that C4 hydroxylation influences the physical and structural properties of DIGs and suggest that syringomycin E interacts with lipid rafts.

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Citations

Dec 13, 2005·Journal of Bioenergetics and Biomembranes·Yuri A KaulinJoseph G Brand
Dec 15, 2015·European Biophysics Journal : EBJ·Svetlana S EfimovaOlga S Ostroumova
Jul 5, 2011·Analytical Biochemistry·Laura Carmona-SalazarMarina Gavilanes-Ruíz
Apr 13, 2005·Microbes and Infection·Leonardo NimrichterLuiz R Travassos
Feb 3, 2005·Yeast
Jan 26, 2020·Plants·Nora A Gutiérrez-NájeraMarina Gavilanes-Ruiz

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