Sphingolipid metabolism is strikingly different between pollen and leaf in Arabidopsis as revealed by compositional and gene expression profiling

Phytochemistry
Kyle D LuttgeharmEdgar B Cahoon

Abstract

Although sphingolipids are essential for male gametophytic development in Arabidopsis thaliana, sphingolipid composition and biosynthetic gene expression have not been previously examined in pollen. In this report, electrospray ionization (ESI)-MS/MS was applied to characterization of sphingolipid compositional profiles in pollen isolated from wild type Arabidopsis Col-0 and a long-chain base (LCB) Δ4 desaturase mutant. Pollen fractions were highly enriched in glucosylceramides (GlcCer) relative to levels previously reported in leaves. Accompanying the loss of the Δ4 unsaturated LCB sphingadiene (d18:2) in the Δ4 desaturase mutant was a 50% reduction in GlcCer concentrations. In addition, pollen glycosylinositolphosphoceramides (GIPCs) were found to have a complex array of N-acetyl-glycosylated GIPCs, including species with up to three pentose units that were absent from leaf GIPCs. Underlying the distinct sphingolipid composition of pollen, genes for key biosynthetic enzymes for GlcCer and d18:2 synthesis and metabolism were more highly expressed in pollen than in leaves or seedlings, including genes for GlcCer synthase (GCS), sphingoid base C-4 hydroxylase 2 (SBH2), LCB Δ8 desaturases (SLD1 and SLD2), and LOH2 ceramide syntha...Continue Reading

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Citations

Apr 2, 2016·Biochimica Et Biophysica Acta·Till Ischebeck
Apr 5, 2016·Journal of Mass Spectrometry : JMS·Corinne BuréJean-Marie Schmitter
Apr 14, 2016·Plant Signaling & Behavior·Julien GronnierSébastien Mongrand
Apr 18, 2016·Biochimica Et Biophysica Acta·Louise V MichaelsonJean-Denis Faure
May 5, 2012·Progress in Lipid Research·Jean-Luc CacasSébastien Mongrand
Jul 28, 2016·The Plant Journal : for Cell and Molecular Biology·Toshiki IshikawaMaki Kawai-Yamada
Jul 8, 2017·The Plant Journal : for Cell and Molecular Biology·Alexander H RotschTill Ischebeck
Jan 9, 2019·Frontiers in Immunology·Åslög Dahl
Aug 28, 2015·The Plant Journal : for Cell and Molecular Biology·Joseph MsanneEdgar B Cahoon
Apr 1, 2021·The Journal of Biological Chemistry·Adiilah Mamode CassimSébastien Mongrand
Nov 23, 2018·Progress in Lipid Research·Adiilah Mamode CassimSébastien Mongrand

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