Sphingolipids mediate formation of mRNA processing bodies during the heat-stress response of Saccharomyces cerevisiae.

The Biochemical Journal
L Ashley CowartY A Hannun

Abstract

Recent work, especially in the yeast Saccharomyces cerevisiae, has demonstrated that mRNA movement from active translation to cytoplasmic granules, termed mRNA'p-bodies' (processing bodies), occurs in concert with the regulation of translation during cell stress. However, the signals regulating p-body formation are poorly defined. Recent results have demonstrated a function for sphingolipids in regulating translation during heat stress, which led to the current hypothesis that p-bodies may form during heat stress in a sphingolipid-dependent manner. In the present study, we demonstrate that mild-heat-stress-induced formation of p-bodies, as determined by localization of a GFP (green fluorescent protein)-tagged Dcp2p and RFP (red fluorescent protein)-tagged Edc3p to discrete cytoplasmic foci. Sphingoid base synthesis was required for this effect, as inhibition of sphingoid base synthesis attenuated formation of these foci during heat stress. Moreover, treatment of yeast with the exogenous sphingoid bases phyto- and dihydro-sphingosine promoted formation of p-bodies in the absence of heat stress, and the lcb4/lcb5 double-deletion yeast, which accumulates high intracellular levels of sphingoid bases, had large clearly defined p-bod...Continue Reading

References

Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J HeitmanM N Hall
Dec 31, 1997·The Journal of Biological Chemistry·R C DicksonR L Lester
Jan 24, 1998·The Journal of Biological Chemistry·G M JenkinsY Hannun
Jul 25, 1998·The Journal of Biological Chemistry·M M NagiecR C Dickson
Apr 15, 2000·The Journal of Biological Chemistry·N ChungL M Obeid
Sep 23, 2000·Chemistry and Physics of Lipids·H Sawai, Y A Hannun
Dec 5, 2000·Molecular Biology of the Cell·A P GaschP O Brown
Jul 27, 2001·The Journal of Biological Chemistry·N ChungL M Obeid
May 16, 2002·The Journal of Biological Chemistry·Yusuf A Hannun, Lina M Obeid
Aug 23, 2002·Functional & Integrative Genomics·Audrey P Gasch, Margaret Werner-Washburne
Jun 6, 2003·Cellular and Molecular Life Sciences : CMLS·G M Jenkins
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Jun 22, 2005·The Journal of Cell Biology·Nancy KedershaPaul Anderson
Dec 31, 2005·Molecular Biology of the Cell·Karsten D MeierHoward Riezman
May 30, 2006·Progress in Lipid Research·Robert C DicksonRobert L Lester
Feb 27, 2007·The Journal of Biological Chemistry·L Ashley Cowart, Yusuf A Hannun
Apr 13, 2007·Molecular Biology of the Cell·Daniela Teixeira, Roy Parker
Nov 5, 2008·The Journal of Cell Biology·J Ross BuchanRoy Parker
Dec 30, 2008·Methods in Enzymology·Tracy Nissan, Roy Parker
Apr 28, 2009·Current Opinion in Cell Biology·Vidya Balagopal, Roy Parker
Jan 13, 2010·Molecular Cell·J Ross Buchan, Roy Parker
Feb 18, 2010·Molecular Systems Biology·L Ashley CowartXinghua Lu

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Citations

Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Jacob VergheseKevin A Morano
Jul 13, 2012·Genetics·Roy Parker
Nov 14, 2013·Chemistry and Physics of Lipids·David J MontefuscoYusuf A Hannun
Jul 27, 2014·Nucleic Acids Research·Tingting QinW Jim Zheng
May 21, 2013·FEBS Letters·Gábor BaloghLászló Vígh
Aug 26, 2020·Neurochemistry International·Libia Catalina Salinas CastellanosCarina Weissmann
Sep 6, 2020·Progress in Lipid Research·João Henrique Tadini Marilhano FabriMaurizio Del Poeta
Nov 19, 2021·Yeast·Tomas GrouslTomas Vomastek

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