Sphingolipids regulate the yeast high-osmolarity glycerol response pathway.

Molecular and Cellular Biology
Mirai TanigawaTatsuya Maeda

Abstract

The yeast high-osmolarity glycerol response (HOG) mitogen-activated protein (MAP) kinase pathway is activated in response to hyperosmotic stress via two independent osmosensing branches, the Sln1 branch and the Sho1 branch. While the mechanism by which the osmosensing machinery activates the downstream MAP kinase cascade has been well studied, the mechanism by which the machinery senses and responds to hyperosmotic stress remains to be clarified. Here we report that inhibition of the de novo sphingolipid synthesis pathway results in activation of the HOG pathway via both branches. Inhibition of ergosterol biosynthesis also induces activation of the HOG pathway. Sphingolipids and sterols are known to be tightly packed together in cell membranes to form partitioned domains called rafts. Raft-enriched detergent-resistant membranes (DRMs) contain both Sln1 and Sho1, and sphingolipid depletion and hyperosmotic stress have similar effects on the osmosensing machinery of the HOG pathway: dissociation of an Sln1-containing protein complex and elevated association of Sho1 with DRMs. These observations reveal the sphingolipid-mediated regulation of the osmosensing machinery of the HOG pathway.

References

Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·M M NagiecR C Dickson
Jul 1, 1996·Nucleic Acids Research·U GüldenerJ H Hegemann
Dec 31, 1997·The Journal of Biological Chemistry·D HaakT Dunn
Jul 17, 1999·Yeast·A Garay-Arroyo, A A Covarrubias
Mar 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·M BagnatK Simons
Jul 27, 2002·Trends in Genetics : TIG·Sean M O'RourkeErin K O'Shea
Jun 18, 2003·The Journal of Cell Biology·Kyohei Umebayashi, Akihiko Nakano
Jun 25, 2003·The Journal of Cell Biology·VladimIr ReiserHaruo Saito
Oct 7, 2004·Current Opinion in Neurobiology·Tamara GolubPico Caroni
Jun 17, 2006·The EMBO Journal·Kazuo TatebayashiHaruo Saito
Apr 5, 2007·Nature Protocols·Ilka WittigHermann Schägger
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Eniko NagyLászló Vígh
Sep 15, 2007·Nature Protocols·Daniel Lingwood, Kai Simons
Jan 18, 2008·Molecular Biology of the Cell·Kenzi NakanoKazuma Tanaka
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Françoise M RoelantsJeremy Thorner
Jan 2, 2010·Science·Daniel Lingwood, Kai Simons

❮ Previous
Next ❯

Citations

Dec 27, 2012·Nucleic Acids Research·Thair JudehDongxiao Zhu
Oct 3, 2012·Genetics·Haruo Saito, Francesc Posas
Nov 14, 2013·Chemistry and Physics of Lipids·David J MontefuscoYusuf A Hannun
Sep 18, 2014·Science Signaling·Jay L Brewster, Michael C Gustin
Aug 22, 2014·Molecular Biology of the Cell·Aline Xavier da Silveira Dos SantosHoward Riezman
Dec 26, 2015·Progress in Lipid Research·Vitor Teixeira, Vítor Costa
Jun 29, 2014·Biochimica Et Biophysica Acta·Pieter SpincemailleKarin Thevissen
Feb 2, 2015·World Journal of Microbiology & Biotechnology·Luis Parmenio Suescún-Bolívar, Patricia Elena Thomé
Aug 20, 2016·Molecular Microbiology·Patrícia Alves de CastroGustavo H Goldman
Dec 13, 2016·International Journal of Molecular Sciences·François HéricourtSabine Carpin
Jul 12, 2018·Molecular Microbiology·Telma S MartinsClara Pereira
Nov 28, 2018·Genes & Development·Kristin L LeskoskeJeremy Thorner
Nov 7, 2018·FEMS Microbiology Letters·Alexandra KonecnaYvetta Gbelska
Aug 2, 2017·Nature Structural & Molecular Biology·Max B FerrettiKatrin Karbstein
Mar 21, 2018·Current Genetics·Motohiro Tani, Kouichi Funato
Jan 30, 2019·Frontiers in Microbiology·João Henrique Tadini Marilhano FabriIran Malavazi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.