Sphingomyelin and cholesterol promote HIV-1 gp41 pretransmembrane sequence surface aggregation and membrane restructuring.

The Journal of Biological Chemistry
Asier Sáez-CiriónJosé L Nieva

Abstract

The interfacial sequence DKWASLWNWFNITNWLWYIK, preceding the transmembrane anchor of gp41 glycoprotein subunit, has been shown to be essential for fusion activity and incorporation into virions. HIV(c), a peptide representing this region, formed lytic pores in liposomes composed of the main lipids occurring in the human immunodeficiency virus, type 1 (HIV-1), envelope, i.e. 1-palmitoyl-2-oleoylphosphatidylcholine (POPC):sphingomyelin (SPM):cholesterol (Chol) (1:1:1 mole ratio), at low (>1:10,000) peptide-to-lipid mole ratio, and promoted the mixing of vesicular lipids at >1:1000 peptide-to-lipid mole ratios. Inclusion of SPM or Chol in POPC membranes had different effects. Whereas SPM sustained pore formation, Chol promoted fusion activity. Even if partitioning into membranes was not affected in the absence of both SPM and Chol, HIV(c) had virtually no effect on POPC vesicles. Conditions described to disturb occurrence of lateral separation of phases in these systems reproduced the high peptide-dose requirements for leakage as found in pure POPC vesicles and inhibited fusion. Surface aggregation assays using rhodamine-labeled peptides demonstrated that SPM and Chol promoted HIV(c) self-aggregation in membranes. Employing head-g...Continue Reading

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Jul 24, 2008·Carlos Julio Montoya Guarin

References

Nov 14, 1985·The New England Journal of Medicine·P S SarinA S Lippa
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R C AloiaL M Gordon
Jun 18, 1985·Biochemistry·H EllensF C Szoka
Jul 7, 1981·Biochemistry·D K StruckR E Pagano
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R C AloiaF C Jensen
Aug 1, 1995·Current Opinion in Structural Biology·L V Chernomordik, J Zimmerberg
Nov 1, 1995·Trends in Biochemical Sciences·Y Shai
Jun 1, 1996·Biophysical Journal·D RapaportY Shai
Oct 29, 1996·Biochimica Et Biophysica Acta·D Marsh
Jul 15, 1998·The Journal of Membrane Biology·D A Brown, E London
Sep 29, 1998·Methods in Enzymology·S H WhiteK Hristova
Oct 6, 1998·The Journal of General Physiology·V I RazinkovF S Cohen
Oct 17, 1998·Methods : a Companion to Methods in Enzymology·P G PiattiL P Villarreal
Jan 8, 1999·Journal of Molecular Biology·I Ben-EfraimY Shai
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·S H White, W C Wimley
Feb 2, 2000·Biophysical Journal·J Bentz
Apr 12, 2000·Biochemistry·W C Wimley, S H White
Apr 20, 2000·The Journal of Biological Chemistry·D A Brown, E London
Apr 25, 2000·Progress in Lipid Research·S Nir, J L Nieva
Oct 12, 2000·The Journal of Biological Chemistry·Y KligerY Shai
Oct 19, 2000·The Journal of Cell Biology·R W Doms, J P Moore
Feb 27, 2001·Biophysical Journal·J M CaaveiroJ M González-Mañas

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Citations

Jun 29, 2004·European Biophysics Journal : EBJ·Cornelia SchroederTse-I Lin
Jun 6, 2003·Microbes and Infection·M Javad AmanSina Bavari
Sep 19, 2003·AIDS Research and Human Retroviruses·Zhaohao LiaoJames E K Hildreth
Mar 3, 2007·AIDS Research and Human Retroviruses·Patrick W MobleyLarry M Gordon
Oct 28, 2006·AIDS Research and Human Retroviruses·Silvia Sánchez-MartínezJosé L Nieva
Oct 11, 2005·Viral Immunology·Caroline GilbertMichel J Tremblay
Jun 2, 2011·Cold Spring Harbor Perspectives in Biology·Maier Lorizate, Hans-Georg Kräusslich
Sep 5, 2008·Journal of Virology·Anneke FunkHüseyin Sirma
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Nathalie Chazal, Denis Gerlier
Jun 21, 2012·The European Physical Journal. E, Soft Matter·F CampeloA Hernández-Machado
Dec 2, 2010·Retrovirology·Shujun LiuZene Matsuda
May 3, 2013·AIDS Research and Therapy·Edwin A HiguitaCarlos J Montoya
Apr 8, 2014·Chemistry and Physics of Lipids·Beatriz ApellánizJosé L Nieva
Sep 7, 2014·Biochimie·Fabrice DumasLaurence Salomé
Aug 2, 2003·Molecular Membrane Biology·Satinder S RawatAnu Puri
Apr 23, 2009·Virus Research·Abdul A Waheed, Eric O Freed
Apr 22, 2008·Biochemical and Biophysical Research Communications·Yanning LuJames P Tam
Jul 16, 2014·Biochimica Et Biophysica Acta·Gregory J HardyStefan Zauscher
Dec 26, 2006·Chemistry and Physics of Lipids·Elmar PrennerKarl Lohner
Jun 25, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Lishuang ZhangYaming Shan
Oct 11, 2012·Immunological Reviews·Jean-Philippe JulienIan A Wilson
May 18, 2011·Journal of Molecular Recognition : JMR·José L NievaMaier Lorizate
Jul 11, 2006·Biophysical Journal·Boris G TenchovDavid P Siegel
Nov 3, 2004·Biochimica Et Biophysica Acta·Richard M Epand
Apr 1, 2006·Progress in Lipid Research·Richard M Epand

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