Sphingomyelin-induced structural modification of native human hemoglobin and its chemically and thermally disrupted secondary structure: A photophysical exploration

Colloids and Surfaces. B, Biointerfaces
Riya SettNikhil Guchhait

Abstract

Sphingomyelin-induced structural modification of Human Hemoglobin (Hb) has been investigated in its native and unfolded conformers that are partially denatured in presence of ∼ 4 M urea, completely denatured in ∼ 8 M urea and thermally disrupted (at ∼ 65 °C) state. The absorption studies unveil ground state complexation between Hb and SM. From steady-state fluorescence and quenching studies alteration of the micro-environments around Trp residues of Hb in above mentioned different cases has been determined. Moreover, lesser exposure of Trp residues to SM in thermally disrupted Hb can be accounted for the exceptionally interesting outcomes in other experiments. The alterations in the time-resolved decay profiles of native Hb, partially and totally chemically denatured as well as thermally disrupted Hb with gradual addition of SM also affirm the amendment of the proteinous micro-environment surrounding Trp residues in a view of FRET between Trp residues and heme group. Wavelength-sensitive emission spectral studies reveal that the protein shows red edge effect in its different conformations in presence and absence of SM. Interestingly, the wavelength-responsive time-resolved study at a constant excitation wavelength demonstrates ...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H A HanssonL Svennerholm
Feb 1, 1991·Journal of Bioenergetics and Biomembranes·A H Merrill
May 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·S R Simon, C R Cantor
Jul 14, 1971·Journal of the American Chemical Society·M C Hsu, R W Woody
Mar 10, 1969·Biochemical and Biophysical Research Communications·H R SloanD S Fredrickson
Nov 1, 1995·Biophysical Journal·P R Maulik, G G Shipley
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·M R Mihailescu, I M Russu
Jan 30, 2002·Cellular and Molecular Life Sciences : CMLS·J Ohanian, V Ohanian
Jan 30, 2002·Luminescence : the Journal of Biological and Chemical Luminescence·Alexander P Demchenko
Feb 9, 2002·Science·Jerry E Squires
Mar 27, 2004·European Biophysics Journal : EBJ·Gerhard M ArtmannChristina Maggakis-Kelemen
Oct 18, 2005·Journal of Colloid and Interface Science·Swati De, Agnishwar Girigoswami
Jul 21, 2006·Biochimica Et Biophysica Acta·Thomas Kolter, Konrad Sandhoff
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Yusuf A Hannun, Lina M Obeid
Feb 10, 2009·The Journal of Physical Chemistry. B·Bhaswati BhattacharyaAnunay Samanta
May 4, 2010·The Journal of Physical Chemistry. B·Mrityunjoy MahatoG B Talapatra
Feb 18, 2011·Biomedical Optics Express·Wei ZhengJianan Y Qu
Mar 5, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bijan Kumar Paul, Nikhil Guchhait
Jan 18, 2012·The Biochemical Journal·Thomas D MullenLina M Obeid
Feb 7, 2014·Physical Chemistry Chemical Physics : PCCP·Indrani JhaPannuru Venkatesu
Apr 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Roberto MonniMajed Chergui
Oct 21, 2015·Advanced Materials·Yi JiaJunbai Li
Nov 2, 2016·Physical Chemistry Chemical Physics : PCCP·Ramakanta MondalSaptarshi Mukherjee
Aug 11, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Le-Le LiYing-Wu Lin
Jan 11, 2020·The Journal of Physical Chemistry. B·Mullah Muhaiminul IslamMoloy Sarkar

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