Sphingomyelin metabolism controls the shape and function of the Golgi cisternae

ELife
Felix CampeloVivek Malhotra

Abstract

The flat Golgi cisterna is a highly conserved feature of eukaryotic cells, but how is this morphology achieved and is it related to its function in cargo sorting and export? A physical model of cisterna morphology led us to propose that sphingomyelin (SM) metabolism at thetrans-Golgi membranes in mammalian cells essentially controls the structural features of a Golgi cisterna by regulating its association to curvature-generating proteins. An experimental test of this hypothesis revealed that affecting SM homeostasis converted flat cisternae into highly curled membranes with a concomitant dissociation of membrane curvature-generating proteins. These data lend support to our hypothesis that SM metabolism controls the structural organization of a Golgi cisterna. Together with our previously presented role of SM in controlling the location of proteins involved in glycosylation and vesicle formation, our data reveal the significance of SM metabolism in the structural organization and function of Golgi cisternae.

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Citations

Sep 7, 2019·The Journal of Cell Biology·Laia BassaganyasJulien Villeneuve
Sep 5, 2019·Journal of the Royal Society, Interface·Morgan Chabanon, Padmini Rangamani
Apr 25, 2018·Nature Cell Biology·Noboru Mizushima
Oct 8, 2020·Soft Matter·Dima BolmatovMaxim O Lavrentovich
Sep 6, 2020·IScience·Yuji SakaiNoboru Mizushima
Nov 11, 2020·ELife·Ishier RaoteFelix Campelo
Mar 12, 2021·Current Opinion in Cell Biology·Bulat R RamazanovJulia von Blume
Mar 13, 2021·Current Opinion in Cell Biology·Pablo LujanFelix Campelo
Mar 18, 2021·Cell Reports·Irene AnastasiaLuca Pellegrini
Nov 23, 2018·Progress in Lipid Research·Adiilah Mamode CassimSébastien Mongrand
Nov 7, 2020·The Journal of Cell Biology·Yuichi WakanaMitsuo Tagaya
May 27, 2021·Archives of Biochemistry and Biophysics·Pablo Lujan, Felix Campelo

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Methods Mentioned

BETA
glycosylation
FCS

Software Mentioned

Leica
ImageJ

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