Sphingomyelin modulates the transbilayer distribution of galactosylceramide in phospholipid membranes.

The Journal of Biological Chemistry
Peter MattjusR E Brown

Abstract

The interrelationships among sphingolipid structure, membrane curvature, and glycosphingolipid transmembrane distribution remain poorly defined despite the emerging importance of sphingolipids in curved regions and vesicle buds of biomembranes. Here, we describe a novel approach to investigate the transmembrane distribution of galactosylceramide in phospholipid small unilamellar vesicles by (13)C NMR spectroscopy. Quantitation of the transbilayer distribution of [6-(13)C]galactosylceramide (99.8% isotopic enrichment) was achieved by exposure of vesicles to the paramagnetic ion, Mn(2+). The data show that [6-(13)C]galactosylceramide prefers (70%) the inner leaflet of phosphatidylcholine vesicles. Increasing the sphingomyelin content of the 1-palmitoyl-2-oleoyl-phosphatidylcholine vesicles shifted galactosylceramide from the inner to the outer leaflet. The amount of galactosylceramide localized in the inner leaflet decreased from 70% in pure 1-palmitoyl-2-oleoyl-phosphatidylcholine vesicles to only 40% in 1-palmitoyl-2-oleoyl-phosphatidylcholine/sphingomyelin (1:2) vesicles. The present study demonstrates that sphingomyelin can dramatically alter the transbilayer distribution of a monohexosylceramide, such as galactosylceramide, ...Continue Reading

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Citations

Jun 15, 2007·European Biophysics Journal : EBJ·Grégory Da CostaArnaud Bondon
Aug 4, 2015·Chemistry and Physics of Lipids·Peter Mattjus
Feb 27, 2007·Biochimica Et Biophysica Acta·Rhoderick E Brown, Peter Mattjus
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May 20, 2021·Physical Review. E·Kayla C SappAlexander J Sodt
May 2, 2006·Chemistry and Physics of Lipids·Michaela Fortelius, Peter Mattjus

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