PMID: 9637373Jun 24, 1998Paper

Sphingomyelinase D activity of brown recluse spider (Loxosceles reclusa) venom as studied by 31P-NMR: effects on the time-course of sphingomyelin hydrolysis

Toxicon : Official Journal of the International Society on Toxinology
M L MerchantC R Geren

Abstract

The time-course for the hydrolysis of the D linkage of chicken egg yolk sphingomyelin in a Triton X-100 mixed micelle and of lysophosphotidylcholine micelles, as catalyzed by brown recluse spider venom and brown recluse spider toxin, was followed by phosphorous-31 nuclear magnetic resonance spectroscopy. The overall rate of hydrolysis of sphingomyelin in mixed micelles was found to be an order of magnitude faster than the hydrolysis of lysophosphotidylcholine. Incorporation of lysophosphotidylcholine into mixed micelles with Triton X-100 inhibited the lipase activity of brown recluse spider venom and brown recluse spider venom toxin. The effects of increased rates of overall reaction were observed with increased temperature and also with decreased ionic strength. The presence of divalent calcium ions was found to be necessary for hydrolytic activity, but only in catalytic amounts (less than 1 mM).

References

Jan 1, 1979·Toxicon : Official Journal of the International Society on Toxinology·Y S JongJ R Heitz
May 1, 1976·Archives of Biochemistry and Biophysics·C R GerenG V Odell
Dec 1, 1974·Infection and Immunity·K D ElgertJ T Barrett
Oct 1, 1973·Toxicon : Official Journal of the International Society on Toxinology·C R GerenG V Odell
Oct 1, 1984·The Journal of Investigative Dermatology·R S ReesL E King
Apr 13, 1982·Biochemistry·A Plückthun, E A Dennis
Jan 1, 1981·Toxicon : Official Journal of the International Society on Toxinology·J L BabcockC R Geren

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Citations

Jun 5, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Greta J Binford, Michael A Wells
Mar 29, 2006·Clinics in Laboratory Medicine·R Brent FurbeeDanyal Ibrahim
Mar 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Katia Cristina BarbaroKen Winkel
Aug 9, 2016·Toxicon : Official Journal of the International Society on Toxinology·Arthur Estanislau DantasEvanguedes Kalapothakis
Oct 27, 2004·Toxicon : Official Journal of the International Society on Toxinology·Paulo Henrique da SilvaSilvio Sanches Veiga
Nov 15, 2019·Toxicon : Official Journal of the International Society on Toxinology·Bárbara Bruna Ribeiro de Oliveira-MendesCarolina Campolina Rebello Horta

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